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James Riley Broach

TitleChair of Biochemistry and Molecular Biology
InstitutionCollege of Medicine
DepartmentBiochemistry and Molecular Biology
Address500 University Drive, Hershey, PA 17033
Mailbox: H171
Phone7175315470

 Overview 
 overview
Dr. Broach is Chair of the Department of Biochemistry and Molecular Biology at Penn State Hershey, Director of the Penn State Institute for Personalized Medicine and Professor Emeritus of Princeton University. He completed his undergraduate studies in Chemistry at Yale University in 1969 and his Ph.D. in Biochemistry from the University of California, Berkeley in 1973, where he also completed a Postdoctoral Fellowship in Medical Physics. Dr. Broach served on the Scientific Review Board of the Frederick Cancer Center of the National Cancer Institute and has served as a member of both the Genetics and the Genomics Study Sections and Chair of the Genomics, Computational Biology and Technology Study Section of the National Institutes of Health. He was Co-Founder and Director of Research for Cadus Pharmaceuticals and sits on the Board of Directors of Cadus Corporation.

Dr. Broach was Professor of Molecular Biology at Princeton University from 1984-2012, where he served as Associate Director of the Lewis Sigler Institute for Integrative Genomics and Co-Director of the Center for Computational Biology. Dr. Broach is a Fellow of the American Academy of Microbiology and the Co-Director of the Life Sciences Research Foundation, a private organization that provides postdoctoral fellowships in the life sciences. Dr. Broach is a member of the Science Board of the Food and Drug Administration and served as Trustee of the University of Medicine and Dentistry of New Jersey and Commissioner on the New Jersey Commission on Cancer Research until 2012. He is a member of the executive committee of the Cancer Biology Training Consortium, a national organization promoting graduate and postdoctoral training in cancer biology. Dr. Broach has published more than 150 articles in the area of molecular biology and holds a number of patents in drug discovery technologies.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Shor E, Fox CA, Broach JR. The yeast environmental stress response regulates mutagenesis induced by proteotoxic stress. PLoS Genet. 2013; 9(8):e1003680.
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  2. Xu YF, Létisse F, Absalan F, Lu W, Kuznetsova E, Brown G, Caudy AA, Yakunin AF, Broach JR, Rabinowitz JD. Nucleotide degradation and ribose salvage in yeast. Mol Syst Biol. 2013; 9:665.
    View in: PubMed
  3. Petrenko N, Chereji RV, McClean MN, Morozov AV, Broach JR. Noise and interlocking signaling pathways promote distinct transcription factor dynamics in response to different stresses. Mol Biol Cell. 2013 Jun; 24(12):2045-57.
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  4. Bravim F, da Silva LF, Souza DT, Lippman SI, Broach JR, Fernandes AA, Fernandes PM. High hydrostatic pressure activates transcription factors involved in Saccharomyces cerevisiae stress tolerance. Curr Pharm Biotechnol. 2012 Dec; 13(15):2712-20.
    View in: PubMed
  5. Broach JR. Nutritional control of growth and development in yeast. Genetics. 2012 Sep; 192(1):73-105.
    View in: PubMed
  6. Bravim F, Lippman SI, da Silva LF, Souza DT, Fernandes AA, Masuda CA, Broach JR, Fernandes PM. High hydrostatic pressure activates gene expression that leads to ethanol production enhancement in a Saccharomyces cerevisiae distillery strain. Appl Microbiol Biotechnol. 2013 Mar; 97(5):2093-107.
    View in: PubMed
  7. Xu YF, Zhao X, Glass DS, Absalan F, Perlman DH, Broach JR, Rabinowitz JD. Regulation of yeast pyruvate kinase by ultrasensitive allostery independent of phosphorylation. Mol Cell. 2012 Oct 12; 48(1):52-62.
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  8. Tsitron J, Ault AD, Broach JR, Morozov AV. Decoding complex chemical mixtures with a physical model of a sensor array. PLoS Comput Biol. 2011 Oct; 7(10):e1002224.
    View in: PubMed
  9. Tolkunov D, Zawadzki KA, Singer C, Elfving N, Morozov AV, Broach JR. Chromatin remodelers clear nucleosomes from intrinsically unfavorable sites to establish nucleosome-depleted regions at promoters. Mol Biol Cell. 2011 Jun 15; 22(12):2106-18.
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  10. Klosinska MM, Crutchfield CA, Bradley PH, Rabinowitz JD, Broach JR. Yeast cells can access distinct quiescent states. Genes Dev. 2011 Feb 15; 25(4):336-49.
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  11. Talarek N, Cameroni E, Jaquenoud M, Luo X, Bontron S, Lippman S, Devgan G, Snyder M, Broach JR, De Virgilio C. Initiation of the TORC1-regulated G0 program requires Igo1/2, which license specific mRNAs to evade degradation via the 5'-3' mRNA decay pathway. Mol Cell. 2010 May 14; 38(3):345-55.
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  12. Shor E, Warren CL, Tietjen J, Hou Z, Müller U, Alborelli I, Gohard FH, Yemm AI, Borisov L, Broach JR, Weinreich M, Nieduszynski CA, Ansari AZ, Fox CA. The origin recognition complex interacts with a subset of metabolic genes tightly linked to origins of replication. PLoS Genet. 2009 Dec; 5(12):e1000755.
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  13. Lippman SI, Broach JR. Protein kinase A and TORC1 activate genes for ribosomal biogenesis by inactivating repressors encoded by Dot6 and its homolog Tod6. Proc Natl Acad Sci U S A. 2009 Nov 24; 106(47):19928-33.
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  14. Zawadzki KA, Morozov AV, Broach JR. Chromatin-dependent transcription factor accessibility rather than nucleosome remodeling predominates during global transcriptional restructuring in Saccharomyces cerevisiae. Mol Biol Cell. 2009 Aug; 20(15):3503-13.
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  15. Freckleton G, Lippman SI, Broach JR, Tavazoie S. Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions. PLoS Genet. 2009 Apr; 5(4):e1000449.
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  16. Zaman S, Lippman SI, Schneper L, Slonim N, Broach JR. Glucose regulates transcription in yeast through a network of signaling pathways. Mol Syst Biol. 2009; 5:245.
    View in: PubMed
  17. Airoldi EM, Huttenhower C, Gresham D, Lu C, Caudy AA, Dunham MJ, Broach JR, Botstein D, Troyanskaya OG. Predicting cellular growth from gene expression signatures. PLoS Comput Biol. 2009 Jan; 5(1):e1000257.
    View in: PubMed
  18. Evans BJ, Wang Z, Broach JR, Oishi S, Fujii N, Peiper SC. Expression of CXCR4, a G-protein-coupled receptor for CXCL12 in yeast identification of new-generation inverse agonists. Methods Enzymol. 2009; 460:399-412.
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  19. Torti FM, Altieri D, Broach J, Fan H, Lotze M, Manfredi J, Matrisian L, Yu D, Giaccia A. Ph.D. Training in cancer biology. Cancer Res. 2008 Nov 15; 68(22):9122-4.
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  20. Tan MP, Smith EN, Broach JR, Floudas CA. Microarray data mining: a novel optimization-based approach to uncover biologically coherent structures. BMC Bioinformatics. 2008; 9:268.
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  21. Zaman S, Lippman SI, Zhao X, Broach JR. How Saccharomyces responds to nutrients. Annu Rev Genet. 2008; 42:27-81.
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  22. Yorimitsu T, Zaman S, Broach JR, Klionsky DJ. Protein kinase A and Sch9 cooperatively regulate induction of autophagy in Saccharomyces cerevisiae. Mol Biol Cell. 2007 Oct; 18(10):4180-9.
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  23. Tan MP, Broach JR, Floudas CA. Evaluation of normalization and pre-clustering issues in a novel clustering approach: global optimum search with enhanced positioning. J Bioinform Comput Biol. 2007 Aug; 5(4):895-913.
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  24. Ramanathan S, Broach JR. Do cells think? Cell Mol Life Sci. 2007 Jul; 64(14):1801-4.
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  25. Urban J, Soulard A, Huber A, Lippman S, Mukhopadhyay D, Deloche O, Wanke V, Anrather D, Ammerer G, Riezman H, Broach JR, De Virgilio C, Hall MN, Loewith R. Sch9 is a major target of TORC1 in Saccharomyces cerevisiae. Mol Cell. 2007 Jun 8; 26(5):663-74.
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  26. McClean MN, Mody A, Broach JR, Ramanathan S. Cross-talk and decision making in MAP kinase pathways. Nat Genet. 2007 Mar; 39(3):409-14.
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  27. Zawadzki K, Broach J. A rapid technique for the visualization of live immobilized yeast cells. J Vis Exp. 2006 Dec; (1):84.
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  28. Guldal CG, Broach J. Assay for adhesion and agar invasion in S. cerevisiae. J Vis Exp. 2006 Dec; (1):64.
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  29. Xu EY, Zawadzki KA, Broach JR. Single-cell observations reveal intermediate transcriptional silencing states. Mol Cell. 2006 Jul 21; 23(2):219-29.
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  30. Houston PL, Broach JR. The dynamics of homologous pairing during mating type interconversion in budding yeast. PLoS Genet. 2006 Jun; 2(6):e98.
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  31. Wang ZX, Broach JR, Peiper SC. Functional expression of CXCR4 in Saccharomyces cerevisiae in the development of powerful tools for the pharmacological characterization of CXCR4. Methods Mol Biol. 2006; 332:115-27.
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  32. Ault AD, Broach JR. Creation of GPCR-based chemical sensors by directed evolution in yeast. Protein Eng Des Sel. 2006 Jan; 19(1):1-8.
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  33. Niida A, Wang Z, Tomita K, Oishi S, Tamamura H, Otaka A, Navenot JM, Broach JR, Peiper SC, Fujii N. Design and synthesis of downsized metastin (45-54) analogs with maintenance of high GPR54 agonistic activity. Bioorg Med Chem Lett. 2006 Jan 1; 16(1):134-7.
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  34. Xu EY, Bi X, Holland MJ, Gottschling DE, Broach JR. Mutations in the nucleosome core enhance transcriptional silencing. Mol Cell Biol. 2005 Mar; 25(5):1846-59.
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  35. Schneper L, Düvel K, Broach JR. Sense and sensibility: nutritional response and signal integration in yeast. Curr Opin Microbiol. 2004 Dec; 7(6):624-30.
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  36. Broach JR. Making the right choice--long-range chromosomal interactions in development. Cell. 2004 Nov 24; 119(5):583-6.
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  37. Santhanam A, Hartley A, Düvel K, Broach JR, Garrett S. PP2A phosphatase activity is required for stress and Tor kinase regulation of yeast stress response factor Msn2p. Eukaryot Cell. 2004 Oct; 3(5):1261-71.
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  38. Jorgensen P, Rupes I, Sharom JR, Schneper L, Broach JR, Tyers M. A dynamic transcriptional network communicates growth potential to ribosome synthesis and critical cell size. Genes Dev. 2004 Oct 15; 18(20):2491-505.
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  39. Wang Y, Pierce M, Schneper L, Güldal CG, Zhang X, Tavazoie S, Broach JR. Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. PLoS Biol. 2004 May; 2(5):E128.
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  40. Houston P, Simon PJ, Broach JR. The Saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination. Genetics. 2004 Mar; 166(3):1187-97.
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  41. Masui T, Doi R, Mori T, Toyoda E, Koizumi M, Kami K, Ito D, Peiper SC, Broach JR, Oishi S, Niida A, Fujii N, Imamura M. Metastin and its variant forms suppress migration of pancreatic cancer cells. Biochem Biophys Res Commun. 2004 Feb 27; 315(1):85-92.
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  42. Schneper L, Krauss A, Miyamoto R, Fang S, Broach JR. The Ras/protein kinase A pathway acts in parallel with the Mob2/Cbk1 pathway to effect cell cycle progression and proper bud site selection. Eukaryot Cell. 2004 Feb; 3(1):108-20.
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  43. Zhang WB, Wang ZX, Murray JL, Fujii N, Broach J, Peiper SC. Functional expression of CXCR4 in S. cerevisiae: development of tools for mechanistic and pharmacologic studies. Ernst Schering Res Found Workshop. 2004; (45):125-52.
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  44. Düvel K, Broach JR. The role of phosphatases in TOR signaling in yeast. Curr Top Microbiol Immunol. 2004; 279:19-38.
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  45. Lin X, Floudas CA, Wang Y, Broach JR. Theoretical and computational studies of the glucose signaling pathways in yeast using global gene expression data. Biotechnol Bioeng. 2003 Dec 30; 84(7):864-86.
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  46. Alvarez Arias D, Navenot JM, Zhang WB, Broach J, Peiper SC. Constitutive activation of CCR5 and CCR2 induced by conformational changes in the conserved TXP motif in transmembrane helix 2. J Biol Chem. 2003 Sep 19; 278(38):36513-21.
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  47. Düvel K, Santhanam A, Garrett S, Schneper L, Broach JR. Multiple roles of Tap42 in mediating rapamycin-induced transcriptional changes in yeast. Mol Cell. 2003 Jun; 11(6):1467-78.
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  48. Schedl P, Broach JR. Making good neighbors: the right fence for the right job. Nat Struct Biol. 2003 Apr; 10(4):241-3.
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  49. Yu Q, Qiu R, Foland TB, Griesen D, Galloway CS, Chiu YH, Sandmeier J, Broach JR, Bi X. Rap1p and other transcriptional regulators can function in defining distinct domains of gene expression. Nucleic Acids Res. 2003 Feb 15; 31(4):1224-33.
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  50. Simon P, Houston P, Broach J. Directional bias during mating type switching in Saccharomyces is independent of chromosomal architecture. EMBO J. 2002 May 1; 21(9):2282-91.
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  51. Zhang WB, Navenot JM, Haribabu B, Tamamura H, Hiramatu K, Omagari A, Pei G, Manfredi JP, Fujii N, Broach JR, Peiper SC. A point mutation that confers constitutive activity to CXCR4 reveals that T140 is an inverse agonist and that AMD3100 and ALX40-4C are weak partial agonists. J Biol Chem. 2002 Jul 5; 277(27):24515-21.
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  52. Haney SA, Xu J, Lee SY, Ma CL, Duzic E, Broach JR, Manfredi JP. Genetic selection in Saccharomyces of mutant mammalian adenylyl cyclases with elevated basal activities. Mol Genet Genomics. 2001 Aug; 265(6):1120-8.
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  53. Nielsen KH, Gredsted L, Broach JR, Willumsen BM. Sensitivity of wild type and mutant ras alleles to Ras specific exchange factors: Identification of factor specific requirements. Oncogene. 2001 Apr 19; 20(17):2091-100.
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  54. Bi X, Broach JR. Chromosomal boundaries in S. cerevisiae. Curr Opin Genet Dev. 2001 Apr; 11(2):199-204.
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  55. Johnston SD, Enomoto S, Schneper L, McClellan MC, Twu F, Montgomery ND, Haney SA, Broach JR, Berman J. CAC3(MSI1) suppression of RAS2(G19V) is independent of chromatin assembly factor I and mediated by NPR1. Mol Cell Biol. 2001 Mar; 21(5):1784-94.
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  56. Wu J, Tolstykh T, Lee J, Boyd K, Stock JB, Broach JR. Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo. EMBO J. 2000 Nov 1; 19(21):5672-81.
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  57. Bi X, Braunstein M, Shei GJ, Broach JR. The yeast HML I silencer defines a heterochromatin domain boundary by directional establishment of silencing. Proc Natl Acad Sci U S A. 1999 Oct 12; 96(21):11934-9.
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  58. Jiang Y, Broach JR. Tor proteins and protein phosphatase 2A reciprocally regulate Tap42 in controlling cell growth in yeast. EMBO J. 1999 May 17; 18(10):2782-92.
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  59. Bi X, Broach JR. UASrpg can function as a heterochromatin boundary element in yeast. Genes Dev. 1999 May 1; 13(9):1089-101.
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  60. Meluh PB, Broach JR. Immunological analysis of yeast chromatin. Methods Enzymol. 1999; 304:414-30.
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  61. Klein C, Paul JI, Sauvé K, Schmidt MM, Arcangeli L, Ransom J, Trueheart J, Manfredi JP, Broach JR, Murphy AJ. Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast. Nat Biotechnol. 1998 Dec; 16(13):1334-7.
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  62. Jiang Y, Davis C, Broach JR. Efficient transition to growth on fermentable carbon sources in Saccharomyces cerevisiae requires signaling through the Ras pathway. EMBO J. 1998 Dec 1; 17(23):6942-51.
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  63. Silverman L, Campbell R, Broach JR. New assay technologies for high-throughput screening. Curr Opin Chem Biol. 1998 Jun; 2(3):397-403.
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  64. Epstein CB, Attiyeh EF, Hobson DA, Silver AL, Broach JR, Levine AJ. p53 mutations isolated in yeast based on loss of transcription factor activity: similarities and differences from p53 mutations detected in human tumors. Oncogene. 1998 Apr 23; 16(16):2115-22.
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  65. Mandala SM, Thornton R, Tu Z, Kurtz MB, Nickels J, Broach J, Menzeleev R, Spiegel S. Sphingoid base 1-phosphate phosphatase: a key regulator of sphingolipid metabolism and stress response. Proc Natl Acad Sci U S A. 1998 Jan 6; 95(1):150-5.
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  66. Bi X, Broach JR. DNA in transcriptionally silent chromatin assumes a distinct topology that is sensitive to cell cycle progression. Mol Cell Biol. 1997 Dec; 17(12):7077-87.
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  67. Szeto L, Fafalios MK, Zhong H, Vershon AK, Broach JR. Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III. Genes Dev. 1997 Aug 1; 11(15):1899-911.
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  68. Holmes SG, Rose AB, Steuerle K, Saez E, Sayegh S, Lee YM, Broach JR. Hyperactivation of the silencing proteins, Sir2p and Sir3p, causes chromosome loss. Genetics. 1997 Mar; 145(3):605-14.
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  69. Szeto L, Broach JR. Role of alpha2 protein in donor locus selection during mating type interconversion. Mol Cell Biol. 1997 Feb; 17(2):751-9.
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  70. Broach JR, Levine AJ. Oncogenes and cell proliferation. Curr Opin Genet Dev. 1997 Feb; 7(1):1-6.
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  71. Broach JR, Thorner J. High-throughput screening for drug discovery. Nature. 1996 Nov 7; 384(6604 Suppl):14-6.
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  72. Manfredi JP, Klein C, Herrero JJ, Byrd DR, Trueheart J, Wiesler WT, Fowlkes DM, Broach JR. Yeast alpha mating factor structure-activity relationship derived from genetically selected peptide agonists and antagonists of Ste2p. Mol Cell Biol. 1996 Sep; 16(9):4700-9.
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  73. Braunstein M, Sobel RE, Allis CD, Turner BM, Broach JR. Efficient transcriptional silencing in Saccharomyces cerevisiae requires a heterochromatin histone acetylation pattern. Mol Cell Biol. 1996 Aug; 16(8):4349-56.
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  74. Holmes SG, Broach JR. Silencers are required for inheritance of the repressed state in yeast. Genes Dev. 1996 Apr 15; 10(8):1021-32.
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  75. Nickels JT, Broach JR. A ceramide-activated protein phosphatase mediates ceramide-induced G1 arrest of Saccharomyces cerevisiae. Genes Dev. 1996 Feb 15; 10(4):382-94.
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  76. Shei GJ, Broach JR. Yeast silencers can act as orientation-dependent gene inactivation centers that respond to environmental signals. Mol Cell Biol. 1995 Jul; 15(7):3496-506.
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  77. Neuman-Silberberg FS, Bhattacharya S, Broach JR. Nutrient availability and the RAS/cyclic AMP pathway both induce expression of ribosomal protein genes in Saccharomyces cerevisiae but by different mechanisms. Mol Cell Biol. 1995 Jun; 15(6):3187-96.
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  78. Weiler KS, Szeto L, Broach JR. Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae. Genetics. 1995 Apr; 139(4):1495-510.
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  79. Bhattacharya S, Chen L, Broach JR, Powers S. Ras membrane targeting is essential for glucose signaling but not for viability in yeast. Proc Natl Acad Sci U S A. 1995 Mar 28; 92(7):2984-8.
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  80. Levine AJ, Broach JR. Oncogenes and cell proliferation. Curr Opin Genet Dev. 1995 Feb; 5(1):1-4.
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  81. Haney SA, Broach JR. Cdc25p, the guanine nucleotide exchange factor for the Ras proteins of Saccharomyces cerevisiae, promotes exchange by stabilizing Ras in a nucleotide-free state. J Biol Chem. 1994 Jun 17; 269(24):16541-8.
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  82. Robinson MK, van Zyl WH, Phizicky EM, Broach JR. TPD1 of Saccharomyces cerevisiae encodes a protein phosphatase 2C-like activity implicated in tRNA splicing and cell separation. Mol Cell Biol. 1994 Jun; 14(6):3634-45.
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  83. Braunstein M, Rose AB, Holmes SG, Allis CD, Broach JR. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. Genes Dev. 1993 Apr; 7(4):592-604.
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  84. Weiler KS, Broach JR. Donor locus selection during Saccharomyces cerevisiae mating type interconversion responds to distant regulatory signals. Genetics. 1992 Dec; 132(4):929-42.
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  85. van Zyl W, Huang W, Sneddon AA, Stark M, Camier S, Werner M, Marck C, Sentenac A, Broach JR. Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae. Mol Cell Biol. 1992 Nov; 12(11):4946-59.
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  86. Quinlan JJ, Nickels JT, Wu WI, Lin YP, Broach JR, Carman GM. The 45- and 104-kDa forms of phosphatidate phosphatase from Saccharomyces cerevisiae are regulated differentially by phosphorylation via cAMP-dependent protein kinase. J Biol Chem. 1992 Sep 5; 267(25):18013-20.
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  87. Russo FD, Scherson I, Broach JR. Direct simulation of yeast 2-microns circle plasmid amplification. J Theor Biol. 1992 Apr 7; 155(3):369-85.
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  88. Broach JR. Ras-regulated signaling processes in Saccharomyces cerevisiae. Curr Opin Genet Dev. 1991 Oct; 1(3):370-7.
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  89. Dubey DD, Davis LR, Greenfeder SA, Ong LY, Zhu JG, Broach JR, Newlon CS, Huberman JA. Evidence suggesting that the ARS elements associated with silencers of the yeast mating-type locus HML do not function as chromosomal DNA replication origins. Mol Cell Biol. 1991 Oct; 11(10):5346-55.
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  90. Garrett S, Menold MM, Broach JR. The Saccharomyces cerevisiae YAK1 gene encodes a protein kinase that is induced by arrest early in the cell cycle. Mol Cell Biol. 1991 Aug; 11(8):4045-52.
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  91. Jones S, Vignais ML, Broach JR. The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras. Mol Cell Biol. 1991 May; 11(5):2641-6.
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  92. Mahoney DJ, Marquardt R, Shei GJ, Rose AB, Broach JR. Mutations in the HML E silencer of Saccharomyces cerevisiae yield metastable inheritance of transcriptional repression. Genes Dev. 1991 Apr; 5(4):605-15.
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  93. Broach JR. RAS genes in Saccharomyces cerevisiae: signal transduction in search of a pathway. Trends Genet. 1991 Jan; 7(1):28-33.
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  94. Rose MD, Broach JR. Cloning genes by complementation in yeast. Methods Enzymol. 1991; 194:195-230.
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  95. Greenwald I, Broach JR. Cell fates in C. elegans: in medias ras. Cell. 1990 Dec 21; 63(6):1113-6.
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  96. Deschenes RJ, Resh MD, Broach JR. Acylation and prenylation of proteins. Curr Opin Cell Biol. 1990 Dec; 2(6):1108-13.
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  97. Fedor-Chaiken M, Deschenes RJ, Broach JR. SRV2, a gene required for RAS activation of adenylate cyclase in yeast. Cell. 1990 Apr 20; 61(2):329-40.
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  98. Broach JR, Deschenes RJ. The function of ras genes in Saccharomyces cerevisiae. Adv Cancer Res. 1990; 54:79-139.
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  99. Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-microns circle-based vectors. Methods Enzymol. 1990; 185:234-79.
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  100. Mahoney DJ, Broach JR. The HML mating-type cassette of Saccharomyces cerevisiae is regulated by two separate but functionally equivalent silencers. Mol Cell Biol. 1989 Nov; 9(11):4621-30.
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  101. Volkert FC, Wilson DW, Broach JR. Deoxyribonucleic acid plasmids in yeasts. Microbiol Rev. 1989 Sep; 53(3):299-317.
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  102. van Zyl WH, Wills N, Broach JR. A general screen for mutant of Saccharomyces cerevisiae deficient in tRNA biosynthesis. Genetics. 1989 Sep; 123(1):55-68.
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  103. Garrett S, Broach J. Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase. Genes Dev. 1989 Sep; 3(9):1336-48.
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  104. Deschenes RJ, Stimmel JB, Clarke S, Stock J, Broach JR. RAS2 protein of Saccharomyces cerevisiae is methyl-esterified at its carboxyl terminus. J Biol Chem. 1989 Jul 15; 264(20):11865-73.
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  105. Armstrong KA, Som T, Volkert FC, Rose A, Broach JR. Propagation and expression of genes in yeast using 2-micron circle vectors. Biotechnology. 1989; 13:165-92.
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  106. Som T, Armstrong KA, Volkert FC, Broach JR. Autoregulation of 2 micron circle gene expression provides a model for maintenance of stable plasmid copy levels. Cell. 1988 Jan 15; 52(1):27-37.
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  107. Marshall M, Mahoney D, Rose A, Hicks JB, Broach JR. Functional domains of SIR4, a gene required for position effect regulation in Saccharomyces cerevisiae. Mol Cell Biol. 1987 Dec; 7(12):4441-52.
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  108. Deschenes RJ, Broach JR. Fatty acylation is important but not essential for Saccharomyces cerevisiae RAS function. Mol Cell Biol. 1987 Jul; 7(7):2344-51.
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  109. Brugge JS, Jarosik G, Andersen J, Queral-Lustig A, Fedor-Chaiken M, Broach JR. Expression of Rous sarcoma virus transforming protein pp60v-src in Saccharomyces cerevisiae cells. Mol Cell Biol. 1987 Jun; 7(6):2180-7.
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  110. Wu LC, Fisher PA, Broach JR. A yeast plasmid partitioning protein is a karyoskeletal component. J Biol Chem. 1987 Jan 15; 262(2):883-91.
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  111. McLeod M, Craft S, Broach JR. Identification of the crossover site during FLP-mediated recombination in the Saccharomyces cerevisiae plasmid 2 microns circle. Mol Cell Biol. 1986 Oct; 6(10):3357-67.
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  112. Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell. 1986 Aug 15; 46(4):541-50.
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  113. Andrews BJ, McLeod M, Broach J, Sadowski PD. Interaction of the FLP recombinase of the Saccharomyces cerevisiae 2 micron plasmid with mutated target sequences. Mol Cell Biol. 1986 Jul; 6(7):2482-9.
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  114. Broach JR. New approaches to a genetic analysis of mitosis. Cell. 1986 Jan 17; 44(1):3-4.
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  115. Volkert FC, Wu LC, Fisher PA, Broach JR. Survival strategies of the yeast plasmid two-micron circle. Basic Life Sci. 1986; 40:375-96.
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  116. Sutton A, Broach JR. Signals for transcription initiation and termination in the Saccharomyces cerevisiae plasmid 2 micron circle. Mol Cell Biol. 1985 Oct; 5(10):2770-80.
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  117. Jayaram M, Sutton A, Broach JR. Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 microns circle. Mol Cell Biol. 1985 Sep; 5(9):2466-75.
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  118. Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M. In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell. 1985 Jan; 40(1):27-36.
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  119. Kataoka T, Powers S, Cameron S, Fasano O, Goldfarb M, Broach J, Wigler M. Functional homology of mammalian and yeast RAS genes. Cell. 1985 Jan; 40(1):19-26.
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  120. Feldman JB, Hicks JB, Broach JR. Identification of sites required for repression of a silent mating type locus in yeast. J Mol Biol. 1984 Oct 5; 178(4):815-34.
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  121. Hicks J, Strathern J, Klar A, Ismail S, Broach J. Structure of the SAD mutation and the location of control sites at silent mating type genes in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Jul; 4(7):1278-85.
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  122. Kataoka T, Powers S, McGill C, Fasano O, Strathern J, Broach J, Wigler M. Genetic analysis of yeast RAS1 and RAS2 genes. Cell. 1984 Jun; 37(2):437-45.
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  123. Powers S, Kataoka T, Fasano O, Goldfarb M, Strathern J, Broach J, Wigler M. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins. Cell. 1984 Mar; 36(3):607-12.
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  124. McLeod M, Volkert F, Broach J. Components of the site-specific recombination system encoded by the yeast plasmid 2-micron circle. Cold Spring Harb Symp Quant Biol. 1984; 49:779-87.
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  125. Jayaram M, Broach JR. Yeast plasmid 2-micron circle promotes recombination within bacterial transposon Tn5. Proc Natl Acad Sci U S A. 1983 Dec; 80(23):7264-8.
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  126. Jayaram M, Li YY, Broach JR. The yeast plasmid 2mu circle encodes components required for its high copy propagation. Cell. 1983 Aug; 34(1):95-104.
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  127. Broach JR. Construction of high copy yeast vectors using 2-microns circle sequences. Methods Enzymol. 1983; 101:307-25.
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  128. Broach JR, Li YY, Feldman J, Jayaram M, Abraham J, Nasmyth KA, Hicks JB. Localization and sequence analysis of yeast origins of DNA replication. Cold Spring Harb Symp Quant Biol. 1983; 47 Pt 2:1165-73.
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  129. Abraham J, Feldman J, Nasmyth KA, Strathern JN, Klar AJ, Broach JR, Hicks JB. Sites required for position-effect regulation of mating-type information in yeast. Cold Spring Harb Symp Quant Biol. 1983; 47 Pt 2:989-98.
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  130. Falco SC, Li Y, Broach JR, Botstein D. Genetic properties of chromosomally integrated 2 mu plasmid DNA in yeast. Cell. 1982 Jun; 29(2):573-84.
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  131. Broach JR, Guarascio VR, Jayaram M. Recombination within the yeast plasmid 2mu circle is site-specific. Cell. 1982 May; 29(1):227-34.
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  132. Broach JR. The yeast plasmid 2 mu circle. Cell. 1982 Feb; 28(2):203-4.
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  133. Broach JR, Friedman L, Sherman F. Correspondence of yeast UAA suppressors to cloned tRNASerUCA genes. J Mol Biol. 1981 Aug 15; 150(3):375-87.
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  134. Klar AJ, Strathern JN, Broach JR, Hicks JB. Regulation of transcription in expressed and unexpressed mating type cassettes of yeast. Nature. 1981 Jan 22; 289(5795):239-44.
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  135. Broach JR, Hicks JB. Replication and recombination functions associated with the yeast plasmid, 2 mu circle. Cell. 1980 Sep; 21(2):501-8.
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  136. Broach JR, Strathern JN, Hicks JB. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. Gene. 1979 Dec; 8(1):121-33.
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  137. Broach JR. Galactose regulation in Saccharomyces cerevisiae. The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated. J Mol Biol. 1979 Jun 15; 131(1):41-53.
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  138. Broach JR, Atkins JF, McGill C, Chow L. Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle. Cell. 1979 Apr; 16(4):827-39.
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  139. Petes TD, Broach JR, Wensink PC, Hereford LM, Fink GR, Botstein D. Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene. 1978 Sep; 4(1):37-49.
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  140. Hopper JE, Broach JR, Rowe LB. Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function. Proc Natl Acad Sci U S A. 1978 Jun; 75(6):2878-82.
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