PSU Profiles
Keywords
Last Name
Institution

Christopher Siedlecki

TitleProfessor
InstitutionCollege of Medicine
DepartmentSurgery
Address500 University Drive Hershey PA 17033
Mailbox: H151
Phone7175315716

 Overview 
 overview
PREFERRED TITLE/ROLE:

Professor of Surgery and Bioengineering

GRADUATE PROGRAM AFFILIATIONS:

Bioengineering

EDUCATION:

Ph.D., Case Western Reserve University, 1996
M.S. in Biomedical Engineering, Case Western Reserve University, 1994
B.S. in Biomedical Engineering, Milwaukee School of Engineering, 1991

NARRATIVE:

Dr. Siedlecki's research interests are centered on elucidating the molecular-level processes involved in the interactions of proteins with biomaterial surfaces. These research activities include determining how the physical and chemical properties of synthetic and natural surfaces (e.g. chemical composition, hydrophilic/hydrophobic balance, surface charge, topography) influence protein structure/function relationships, and the development of novel strategies for synthesis and modification of biomaterials. One area of particular interest is the role of the biomaterial interface on the initial events that promote the formation of surface-induced thrombus on implanted cardiovascular devices. Thrombogenesis is influenced by a series of poorly understood interactions occurring at the blood/biomaterial interface, primarily involving adsorbed and circulating proteins, platelets, and the biomaterial itself. When a biomaterial is placed into contact with blood, a layer of proteins is rapidly adsorbed on the surface. This protein layer may trigger thrombosis through either of 2 pathways. The first of these involves adhesive proteins such as fibrinogen and von Willebrand Factor. These proteins mediate the adhesion and activation of circulating platelets. The second pathway involves Hageman Factor (human coagulation factor XII) and initiates the contact activation pathway of the coagulation cascade.

It has been demonstrated that surface properties of the underlying biomaterial substrate are important in both the nature and extent of thrombogenesis. However, little is known about the underlying mechanisms behind this phenomena. We utilize a variety of surface techniques and in-vivo and in-vitro biological measurements in order to more clearly define what are the roles of surface chemistry, surface functionality, and surface energy in thrombogenesis in order to provide rationale for further development of blood-contacting materials.

New efforts in the lab have focused on the use of bionanotechnologyto alter the response of blood to materials. Preliminary results suggest that both blood coagulation (the protien response) and platelet adhesion can be altered by changing the properties of biomedical materials at the sub-micron level. These studies focus on the applied aspects of biomaterials.

Because our work is both physical and biological in nature, many of my group's publications are not referenced in pubmed. A complete list of publications from my group can be found on my laboratory home page listed below.


 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
List All   |   Timeline
  1. Golas A, Pitakjakpipop H, Rahn MS, Siedlecki CA, Vogler EA. Enzymes produced by autoactivation of blood factor XII in buffer: A contribution from the Hematology at Biomaterial Interfaces Research Group. Biomaterials. 2015 Jan; 37:1-12.
    View in: PubMed
  2. Xu LC, Bauer JW, Siedlecki CA. Proteins, platelets, and blood coagulation at biomaterial interfaces. Colloids Surf B Biointerfaces. 2014 Dec 1; 124:49-68.
    View in: PubMed
  3. Navitsky MA, Taylor JO, Smith AB, Slattery MJ, Deutsch S, Siedlecki CA, Manning KB. Platelet adhesion to polyurethane urea under pulsatile flow conditions. Artif Organs. 2014 Dec; 38(12):1046-53.
    View in: PubMed
  4. Xu LC, Siedlecki CA. Staphylococcus epidermidis adhesion on hydrophobic and hydrophilic textured biomaterial surfaces. Biomed Mater. 2014 Jun; 9(3):035003.
    View in: PubMed
  5. Ozdemir T, Xu LC, Siedlecki C, Brown JL. Substrate curvature sensing through Myosin IIa upregulates early osteogenesis. Integr Biol (Camb). 2013 Nov; 5(11):1407-16.
    View in: PubMed
  6. Golas A, Yeh CH, Pitakjakpipop H, Siedlecki CA, Vogler EA. A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions. Biomaterials. 2013 Jan; 34(3):607-20.
    View in: PubMed
  7. Lim JY, Siedlecki CA, Donahue HJ. Nanotopographic cell culture substrate: polymer-demixed nanotextured films under cell culture conditions. Biores Open Access. 2012 Oct; 1(5):252-5.
    View in: PubMed
  8. Weiss WJ, Carney EL, Clark JB, Peterson R, Cooper TK, Nifong TP, Siedlecki CA, Hicks D, Doxtater B, Lukic B, Yeager E, Reibson J, Cysyk J, Rosenberg G, Pierce WS. Chronic in vivo testing of the Penn State infant ventricular assist device. ASAIO J. 2012 Jan-Feb; 58(1):65-72.
    View in: PubMed
  9. Golas A, Yeh CH, Siedlecki CA, Vogler EA. Amidolytic, procoagulant, and activation-suppressing proteins produced by contact activation of blood factor XII in buffer solution. Biomaterials. 2011 Dec; 32(36):9747-57.
    View in: PubMed
  10. Xing Y, Gu Y, Xu LC, Siedlecki CA, Donahue HJ, You J. Effects of membrane cholesterol depletion and GPI-anchored protein reduction on osteoblastic mechanotransduction. J Cell Physiol. 2011 Sep; 226(9):2350-9.
    View in: PubMed
  11. Xu LC, Siedlecki CA. Submicron-textured biomaterial surface reduces staphylococcal bacterial adhesion and biofilm formation. Acta Biomater. 2012 Jan; 8(1):72-81.
    View in: PubMed
  12. Soman P, Siedlecki CA. Effects of protein solution composition on the time-dependent functional activity of fibrinogen on surfaces. Langmuir. 2011 Sep 6; 27(17):10814-9.
    View in: PubMed
  13. Xu LC, Siedlecki CA. Microphase separation structure influences protein interactions with poly(urethane urea) surfaces. J Biomed Mater Res A. 2010 Jan; 92(1):126-36.
    View in: PubMed
  14. Xu LC, Runt J, Siedlecki CA. Dynamics of hydrated polyurethane biomaterials: Surface microphase restructuring, protein activity and platelet adhesion. Acta Biomater. 2010 Jun; 6(6):1938-47.
    View in: PubMed
  15. Golas A, Parhi P, Dimachkie ZO, Siedlecki CA, Vogler EA. Surface-energy dependent contact activation of blood factor XII. Biomaterials. 2010 Feb; 31(6):1068-79.
    View in: PubMed
  16. Chatterjee K, Guo Z, Vogler EA, Siedlecki CA. Contributions of contact activation pathways of coagulation factor XII in plasma. J Biomed Mater Res A. 2009 Jul; 90(1):27-34.
    View in: PubMed
  17. Chatterjee K, Thornton JL, Bauer JW, Vogler EA, Siedlecki CA. Moderation of prekallkrein-factor XII interactions in surface activation of coagulation by protein-adsorption competition. Biomaterials. 2009 Oct; 30(28):4915-20.
    View in: PubMed
  18. Xu LC, Siedlecki CA. Atomic force microscopy studies of the initial interactions between fibrinogen and surfaces. Langmuir. 2009 Apr 9; 25(6):3675-81.
    View in: PubMed
  19. Agnihotri A, Soman P, Siedlecki CA. AFM measurements of interactions between the platelet integrin receptor GPIIbIIIa and fibrinogen. Colloids Surf B Biointerfaces. 2009 Jun 1; 71(1):138-47.
    View in: PubMed
  20. Vogler EA, Siedlecki CA. Contact activation of blood-plasma coagulation. Biomaterials. 2009 Apr; 30(10):1857-69.
    View in: PubMed
  21. Soman P, Rice Z, Siedlecki CA. Measuring the time-dependent functional activity of adsorbed fibrinogen by atomic force microscopy. Langmuir. 2008 Aug 19; 24(16):8801-6.
    View in: PubMed
  22. Soman P, Rice Z, Siedlecki CA. Immunological identification of fibrinogen in dual-component protein films by AFM imaging. Micron. 2008 Oct; 39(7):832-42.
    View in: PubMed
  23. Zhuo R, Siedlecki CA, Vogler EA. Competitive-protein adsorption in contact activation of blood factor XII. Biomaterials. 2007 Oct; 28(30):4355-69.
    View in: PubMed
  24. Milner KR, Siedlecki CA. Submicron poly(L-lactic acid) pillars affect fibroblast adhesion and proliferation. J Biomed Mater Res A. 2007 Jul; 82(1):80-91.
    View in: PubMed
  25. Hansen JC, Lim JY, Xu LC, Siedlecki CA, Mauger DT, Donahue HJ. Effect of surface nanoscale topography on elastic modulus of individual osteoblastic cells as determined by atomic force microscopy. J Biomech. 2007; 40(13):2865-71.
    View in: PubMed
  26. Xu LC, Siedlecki CA. Effects of surface wettability and contact time on protein adhesion to biomaterial surfaces. Biomaterials. 2007 Aug; 28(22):3273-83.
    View in: PubMed
  27. Milner KR, Siedlecki CA. Fibroblast response is enhanced by poly(L-lactic acid) nanotopography edge density and proximity. Int J Nanomedicine. 2007; 2(2):201-11.
    View in: PubMed
  28. Xu LC, Soman P, Runt J, Siedlecki CA. Characterization of surface microphase structures of poly(urethane urea) biomaterials by nanoscale indentation with AFM. J Biomater Sci Polym Ed. 2007; 18(4):353-68.
    View in: PubMed
  29. Lim JY, Dreiss AD, Zhou Z, Hansen JC, Siedlecki CA, Hengstebeck RW, Cheng J, Winograd N, Donahue HJ. The regulation of integrin-mediated osteoblast focal adhesion and focal adhesion kinase expression by nanoscale topography. Biomaterials. 2007 Apr; 28(10):1787-97.
    View in: PubMed
  30. Chatterjee K, Vogler EA, Siedlecki CA. Procoagulant activity of surface-immobilized Hageman factor. Biomaterials. 2006 Nov; 27(33):5643-50.
    View in: PubMed
  31. Yamanaka H, Rosenberg G, Weiss WJ, Snyder AJ, Zapanta CM, Siedlecki CA. Short-term in vivo studies of surface thrombosis in a left ventricular assist system. ASAIO J. 2006 May-Jun; 52(3):257-65.
    View in: PubMed
  32. Zhuo R, Siedlecki CA, Vogler EA. Autoactivation of blood factor XII at hydrophilic and hydrophobic surfaces. Biomaterials. 2006 Aug; 27(24):4325-32.
    View in: PubMed
  33. Milner KR, Snyder AJ, Siedlecki CA. Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials. J Biomed Mater Res A. 2006 Mar 1; 76(3):561-70.
    View in: PubMed
  34. Agnihotri A, Garrett JT, Runt J, Siedlecki CA. Atomic force microscopy visualization of poly(urethane urea) microphase rearrangements under aqueous environment. J Biomater Sci Polym Ed. 2006; 17(1-2):227-38.
    View in: PubMed
  35. Miller R, Guo Z, Vogler EA, Siedlecki CA. Plasma coagulation response to surfaces with nanoscale chemical heterogeneity. Biomaterials. 2006 Jan; 27(2):208-15.
    View in: PubMed
  36. Lim JY, Hansen JC, Siedlecki CA, Hengstebeck RW, Cheng J, Winograd N, Donahue HJ. Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: effect of nanotopography, surface chemistry, and wettability. Biomacromolecules. 2005 Nov-Dec; 6(6):3319-27.
    View in: PubMed
  37. Milner KR, Siedlecki CA, Snyder AJ. Development of novel submicron textured polyether(urethane urea) for decreasing platelet adhesion. ASAIO J. 2005 Sep-Oct; 51(5):578-84.
    View in: PubMed
  38. Yamanaka H, Rosenberg G, Weiss WJ, Snyder AJ, Zapanta CM, Siedlecki CA. Multiscale analysis of surface thrombosis in vivo in a left ventricular assist system. ASAIO J. 2005 Sep-Oct; 51(5):567-77.
    View in: PubMed
  39. Guo Z, Bussard KM, Chatterjee K, Miller R, Vogler EA, Siedlecki CA. Mathematical modeling of material-induced blood plasma coagulation. Biomaterials. 2006 Feb; 27(5):796-806.
    View in: PubMed
  40. Hussain MA, Agnihotri A, Siedlecki CA. AFM imaging of ligand binding to platelet integrin alphaIIbbeta3 receptors reconstituted into planar lipid bilayers. Langmuir. 2005 Jul 19; 21(15):6979-86.
    View in: PubMed
  41. Zhuo R, Miller R, Bussard KM, Siedlecki CA, Vogler EA. Procoagulant stimulus processing by the intrinsic pathway of blood plasma coagulation. Biomaterials. 2005 Jun; 26(16):2965-73.
    View in: PubMed
  42. Krishnan A, Wilson A, Sturgeon J, Siedlecki CA, Vogler EA. Liquid-vapor interfacial tension of blood plasma, serum and purified protein constituents thereof. Biomaterials. 2005 Jun; 26(17):3445-53.
    View in: PubMed
  43. Lim JY, Hansen JC, Siedlecki CA, Runt J, Donahue HJ. Human foetal osteoblastic cell response to polymer-demixed nanotopographic interfaces. J R Soc Interface. 2005 Mar 22; 2(2):97-108.
    View in: PubMed
  44. Agnihotri A, Siedlecki CA. Adhesion mode atomic force microscopy study of dual component protein films. Ultramicroscopy. 2005 Mar; 102(4):257-68.
    View in: PubMed
  45. Agnihotri A, Siedlecki CA. Time-dependent conformational changes in fibrinogen measured by atomic force microscopy. Langmuir. 2004 Sep 28; 20(20):8846-52.
    View in: PubMed
  46. Krishnan A, Siedlecki CA, Vogler EA. Mixology of protein solutions and the Vroman effect. Langmuir. 2004 Jun 8; 20(12):5071-8.
    View in: PubMed
  47. Krishnan A, Sturgeon J, Siedlecki CA, Vogler EA. Scaled interfacial activity of proteins at the liquid-vapor interface. J Biomed Mater Res A. 2004 Mar 1; 68(3):544-57.
    View in: PubMed
  48. Hussain MA, Siedlecki CA. The platelet integrin alpha(IIb) beta(3) imaged by atomic force microscopy on model surfaces. Micron. 2004; 35(7):565-73.
    View in: PubMed
  49. Siedlecki CA, Wang IW, Higashi JM, Kottke-Marchant K, Marchant RE. Platelet-derived microparticles on synthetic surfaces observed by atomic force microscopy and fluorescence microscopy. Biomaterials. 1999 Aug; 20(16):1521-9.
    View in: PubMed
  50. Holland NB, Siedlecki CA, Marchant RE. Intermolecular force mapping of platelet surfaces on collagen substrata. J Biomed Mater Res. 1999 Jun 5; 45(3):167-74.
    View in: PubMed
  51. Siedlecki CA, Marchant RE. Atomic force microscopy for characterization of the biomaterial interface. Biomaterials. 1998 Mar; 19(4-5):441-54.
    View in: PubMed
  52. Marchant RE, Barb MD, Shainoff JR, Eppell SJ, Wilson DL, Siedlecki CA. Three dimensional structure of human fibrinogen under aqueous conditions visualized by atomic force microscopy. Thromb Haemost. 1997 Jun; 77(6):1048-51.
    View in: PubMed
  53. Siedlecki CA, Eppell SJ, Marchant RE. Interactions of human von Willebrand factor with a hydrophobic self-assembled monolayer studied by atomic force microscopy. J Biomed Mater Res. 1994 Sep; 28(9):971-80.
    View in: PubMed
Siedlecki's Networks
Click the "See All" links for more information and interactive visualizations!
Concepts
_
Co-Authors
_
Similar People
_
Same Department
Physical Neighbors
_