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Michael Katzman

TitleProfessor
InstitutionCollege of Medicine
DepartmentMedicine
Address500 University Drive Hershey PA 17033
Phone7175318881

 Overview 
 overview
PREFERRED TITLE/ROLE:

Physician, Professor of Medicine (Infectious Diseases) and Microbiology & Immunology
Distinguished Educator, Penn State College of Medicine

OTHER CURRENT TITLES/ROLES:

Director, Antimicrobial Stewardship Program
President, Society of Distinguished Educators
Infectious Diseases Thread Leader for Medical Student Curriculum
Clinical Skills Instructor/Advisor in Waldhausen Society

SELECTED PAST TITLES/ROLES:

Vice-Chair, Biological Safety and Recombinant DNA Committee (1993 - 2011)
Chair, Hospital Infection Control Committee (1993 - 2011)
Faculty Organization Officer (Parliamentarian 2006-2010; President-Elect 2010-2011; President 2011-2013; Immediate Past-President 2013-2014)
Co-Director, Biological Basis of Disease First-Year Medical Student Course (2011-2013)

GRADUATE PROGRAM AFFILIATIONS:

Microbiology and Immunology, MD/PhD Degree Program, Biomedical Sciences (BMS) Graduate Program, Cell and Molecular Biology Graduate Program, Molecular Medicine Intercollege Graduate Program of the Huck Institutes of the Life Sciences

EDUCATION:

Postdoctoral Research Fellowship, Biochemistry and Medicine, Case Western Reserve University (1986-1989)
Fellowship, Infectious Diseases, University Hospitals of Cleveland and Cleveland Veterans Administration Hospital (1984-1989)
Residency, Internal Medicine, University Hospitals of Cleveland and Cleveland Veterans Administration Hospital (1981-1984)
M.D., Columbia University College of Physicians & Surgeons (1977-1981)
A.B., Columbia College, Columbia University (1973-1977)

NARRATIVE:

For more than 2 decades, our laboratory studied the structure and function of the retroviral integrase enzyme that is responsible for catalyzing the permanent integration of a copy of the viral genome into the DNA of an infected cell. We have worked with the integrases of human immunodeficiency virus (HIV), avian sarcoma viruses, and visna virus with the goal of understanding the mechanism of integration so as to develop new ways to prevent or treat the acquired immunodeficiency syndrome (AIDS), but this work is also relevant to certain other human diseases, some animal models of cancer, and gene therapy.

In particular, several years ago I participated in the first purification of an enzymatically active retroviral integrase from a bacterial expression system and developed an assay that is used throughout the world to study this enzyme. After establishing my own laboratory, we created the first set of functional chimeric lentiviral integrases by making novel proteins partly from HIV and partly from a sheep virus; these studies identified the parts of integrase that interact with viral DNA and with human DNA, while also identifying the parts of viral DNA that interact with integrase. We then characterized the largest set (to that point) of HIV integrases derived from infected patients; these studies identified an amino acid in the middle of the protein that likely contacts human DNA during integration. Subsequently, we made several mutations at the position identified by the human samples to yield novel integrases that were improved at acting on viral DNA but impaired at acting on cellular DNA, the first mutants to separate the two biologically relevant actions of integrase in this way. More recent work tried to exploit these properties to define the cellular-DNA and viral-DNA binding sites on the integrase protein. In another project, we explored the potential of stimulating the ability of integrase to nick DNA nonspecifically for a novel antiviral strategy of using integrase to damage viral DNA. Our focus on integrase has contributed to the current model of retroviral integration and will enhance efforts to target specific actions or domains of integrase for clinical therapy. The recent introduction of integrase inhibitors into clinical practice for treating HIV infection has been particularly gratifying.

With the evolution of my career, I have now shifted focus to the appropriate usage of antimicrobial drugs in the healthcare setting, and in 2011 we established a successful Antimicrobial Stewardship Program at Penn State Hershey Medical Center.



 Bibliographic 
 selected publications
Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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  1. Katzman M. Isolation without contact. Infect Control Hosp Epidemiol. 2014 Dec; 35(12):1549.
    View in: PubMed
  2. Katzman M. A piece of my mind. The clock doctor. JAMA. 2013 Apr 3; 309(13):1357-8.
    View in: PubMed
  3. Ealy JB, Sudol M, Krzeminski J, Amin S, Katzman M. Alternative nucleophilic substrates for the endonuclease activities of human immunodeficiency virus type 1 integrase. Virology. 2012 Nov 10; 433(1):149-56.
    View in: PubMed
  4. Sudol M, Fritz JL, Tran M, Robertson GP, Ealy JB, Katzman M. Evaluation of a system to screen for stimulators of non-specific DNA nicking by HIV-1 integrase: application to a library of 50,000 compounds. Antivir Chem Chemother. 2011; 22(2):67-74.
    View in: PubMed
  5. Sudol M, Tran M, Nowak MG, Flanagan JM, Robertson GP, Katzman M. A nonradioactive plate-based assay for stimulators of nonspecific DNA nicking by HIV-1 integrase and other nucleases. Anal Biochem. 2010 Jan 15; 396(2):223-30.
    View in: PubMed
  6. Nowak MG, Sudol M, Lee NE, Konsavage WM, Katzman M. Identifying amino acid residues that contribute to the cellular-DNA binding site on retroviral integrase. Virology. 2009 Jun 20; 389(1-2):141-8.
    View in: PubMed
  7. Konsavage WM, Sudol M, Katzman M. Effects of varying the spacing within the D,D-35-E motif in the catalytic region of retroviral integrase. Virology. 2008 Sep 30; 379(2):223-33.
    View in: PubMed
  8. Konsavage WM, Sudol M, Lee NE, Katzman M. Retroviral integrases that are improved for processing but impaired for joining. Virus Res. 2007 May; 125(2):198-210.
    View in: PubMed
  9. Konsavage WM, Burkholder S, Sudol M, Harper AL, Katzman M. A substitution in rous sarcoma virus integrase that separates its two biologically relevant enzymatic activities. J Virol. 2005 Apr; 79(8):4691-9.
    View in: PubMed
  10. Harper AL, Sudol M, Katzman M. An amino acid in the central catalytic domain of three retroviral integrases that affects target site selection in nonviral DNA. J Virol. 2003 Mar; 77(6):3838-45.
    View in: PubMed
  11. Katzman M, Harper AL, Sudol M, Skinner LM, Eyster ME. Activity of HIV-1 integrases recovered from subjects with varied rates of disease progression. J Acquir Immune Defic Syndr. 2001 Nov 1; 28(3):203-10.
    View in: PubMed
  12. Harper AL, Skinner LM, Sudol M, Katzman M. Use of patient-derived human immunodeficiency virus type 1 integrases to identify a protein residue that affects target site selection. J Virol. 2001 Aug; 75(16):7756-62.
    View in: PubMed
  13. Skinner LM, Sudol M, Harper AL, Katzman M. Nucleophile selection for the endonuclease activities of human, ovine, and avian retroviral integrases. J Biol Chem. 2001 Jan 5; 276(1):114-24.
    View in: PubMed
  14. Morgan AL, Katzman M. Subterminal viral DNA nucleotides as specific recognition signals for human immunodeficiency virus type 1 and visna virus integrases under magnesium-dependent conditions. J Gen Virol. 2000 Mar; 81(Pt 3):839-49.
    View in: PubMed
  15. Katzman M, Sudol M, Pufnock JS, Zeto S, Skinner LM. Mapping target site selection for the non-specific nuclease activities of retroviral integrase. Virus Res. 2000 Jan; 66(1):87-100.
    View in: PubMed
  16. Katzman M, Katz RA. Substrate recognition by retroviral integrases. Adv Virus Res. 1999; 52:371-95.
    View in: PubMed
  17. Skinner LM, Lamers SL, Sanders JC, Eyster ME, Goodenow MM, Katzman M. Analysis of a large collection of natural HIV-1 integrase sequences, including those from long-term nonprogressors. J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Oct 1; 19(2):99-110.
    View in: PubMed
  18. Katzman M, Sudol M. Mapping viral DNA specificity to the central region of integrase by using functional human immunodeficiency virus type 1/visna virus chimeric proteins. J Virol. 1998 Mar; 72(3):1744-53.
    View in: PubMed
  19. Katzman M, Sudol M. Influence of subterminal viral DNA nucleotides on differential susceptibility to cleavage by human immunodeficiency virus type 1 and visna virus integrases. J Virol. 1996 Dec; 70(12):9069-73.
    View in: PubMed
  20. Katzman M, Sudol M. Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases. J Virol. 1996 Apr; 70(4):2598-604.
    View in: PubMed
  21. Katzman M, Sudol M. Mapping domains of retroviral integrase responsible for viral DNA specificity and target site selection by analysis of chimeras between human immunodeficiency virus type 1 and visna virus integrases. J Virol. 1995 Sep; 69(9):5687-96.
    View in: PubMed
  22. Fiscus SA, DeGruttola V, Gupta P, Katzenstein DA, Meyer WA, LoFaro ML, Katzman M, Ragni MV, Reichelderfer PS, Coombs RW. Human immunodeficiency virus type 1 quantitative cell microculture as a measure of antiviral efficacy in a multicenter clinical trial. J Infect Dis. 1995 Feb; 171(2):305-11.
    View in: PubMed
  23. Katzman M, Sudol M. In vitro activities of purified visna virus integrase. J Virol. 1994 Jun; 68(6):3558-69.
    View in: PubMed
  24. Shafer RW, Kozal MJ, Winters MA, Iversen AK, Katzenstein DA, Ragni MV, Meyer WA, Gupta P, Rasheed S, Coombs R, et al. Combination therapy with zidovudine and didanosine selects for drug-resistant human immunodeficiency virus type 1 strains with unique patterns of pol gene mutations. J Infect Dis. 1994 Apr; 169(4):722-9.
    View in: PubMed
  25. Katzman M. Use of oil overlays in "oil-free" PCR technology. Biotechniques. 1993 Jan; 14(1):36-40.
    View in: PubMed
  26. O'Bryan TA, Whitener CJ, Katzman M, Appelbaum PC. Hepatobiliary infections caused by Haemophilus species. Clin Infect Dis. 1992 Oct; 15(4):716-9.
    View in: PubMed
  27. Cobrinik D, Aiyar A, Ge Z, Katzman M, Huang H, Leis J. Overlapping retrovirus U5 sequence elements are required for efficient integration and initiation of reverse transcription. J Virol. 1991 Jul; 65(7):3864-72.
    View in: PubMed
  28. Katzman M, Mack JP, Skalka AM, Leis J. A covalent complex between retroviral integrase and nicked substrate DNA. Proc Natl Acad Sci U S A. 1991 Jun 1; 88(11):4695-9.
    View in: PubMed
  29. Katzman M, Katz RA, Skalka AM, Leis J. The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration. J Virol. 1989 Dec; 63(12):5319-27.
    View in: PubMed
  30. Wiest PM, Flanigan T, Salata RA, Shlaes DM, Katzman M, Lederman MM. Serious infectious complications of corticosteroid therapy for COPD. Chest. 1989 Jun; 95(6):1180-4.
    View in: PubMed
  31. Terry R, Soltis DA, Katzman M, Cobrinik D, Leis J, Skalka AM. Properties of avian sarcoma-leukosis virus pp32-related pol-endonucleases produced in Escherichia coli. J Virol. 1988 Jul; 62(7):2358-65.
    View in: PubMed
  32. Kaminski HJ, Katzman M, Wiest PM, Ellner JJ, Gifford DR, Rackley R, Iskandar SS, Lederman MM. Cardiomyopathy associated with the acquired immune deficiency syndrome. J Acquir Immune Defic Syndr. 1988; 1(2):105-10.
    View in: PubMed
  33. Katzman M, Lederman MM, Spagnuolo PJ. Fibronectin and cellular cytotoxicity: evidence against a role for fibronectin in natural killer activity. J Lab Clin Med. 1987 Jul; 110(1):75-82.
    View in: PubMed
  34. Katzman M, Carey JT, Elmets CA, Jacobs GH, Lederman MM. Molluscum contagiosum and the acquired immunodeficiency syndrome: clinical and immunological details of two cases. Br J Dermatol. 1987 Jan; 116(1):131-8.
    View in: PubMed
  35. Katzman M, Lederman MM. Defective postbinding lysis underlies the impaired natural killer activity in factor VIII-treated, human T lymphotropic virus type III seropositive hemophiliacs. J Clin Invest. 1986 Apr; 77(4):1057-62.
    View in: PubMed
  36. Katzman M, Elmets CA, Lederman MM. Molluscum contagiosum and the acquired immunodeficiency syndrome. Ann Intern Med. 1985 Mar; 102(3):413-4.
    View in: PubMed
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