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James E Hopper
Title Professor Emeritus
Institution College of Medicine
Department Biochemistry and Molecular Biology
Address 500 University Drive, Hershey, PA 17033
Fax 7175317072
Email
Background
PREFERRED TITLE/ROLE:

Professor Emeritus of Biochemistry and Molecular Biology

EDUCATION:

Ph.D.
Publications
1. Egriboz O, Jiang F, Hopper JE. Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80. Genetics. 2011 Nov; 189(3):825-36.
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2. Jiang F, Frey BR, Evans ML, Friel JC, Hopper JE. Gene activation by dissociation of an inhibitor from a transcriptional activation domain. Mol Cell Biol. 2009 Oct; 29(20):5604-10.
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3. Diep CQ, Tao X, Pilauri V, Losiewicz M, Blank TE, Hopper JE. Genetic evidence for sites of interaction between the Gal3 and Gal80 proteins of the Saccharomyces cerevisiae GAL gene switch. Genetics. 2008 Feb; 178(2):725-36.
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4. Diep CQ, Peng G, Bewley M, Pilauri V, Ropson I, Hopper JE. Intragenic suppression of Gal3C interaction with Gal80 in the Saccharomyces cerevisiae GAL gene switch. Genetics. 2006 Jan; 172(1):77-87.
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5. Meehan WJ, Samant RS, Hopper JE, Carrozza MJ, Shevde LA, Workman JL, Eckert KA, Verderame MF, Welch DR. Breast cancer metastasis suppressor 1 (BRMS1) forms complexes with retinoblastoma-binding protein 1 (RBP1) and the mSin3 histone deacetylase complex and represses transcription. J Biol Chem. 2004 Jan 9; 279(2):1562-9.
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6. Adams CA, Kar SR, Hopper JE, Fried MG. Self-association of the amino-terminal domain of the yeast TATA-binding protein. J Biol Chem. 2004 Jan 9; 279(2):1376-82.
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7. Levinson H, Hopper JE, Ehrlich HP. Overexpression of integrin alphav promotes human osteosarcoma cell populated collagen lattice contraction and cell migration. J Cell Physiol. 2002 Nov; 193(2):219-24.
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8. Sandt CH, Hopper JE, Hill CW. Activation of prophage eib genes for immunoglobulin-binding proteins by genes from the IbrAB genetic island of Escherichia coli ECOR-9. J Bacteriol. 2002 Jul; 184(13):3640-8.
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9. Peng G, Hopper JE. Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proc Natl Acad Sci U S A. 2002 Jun 25; 99(13):8548-53.
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10. Carrozza MJ, John S, Sil AK, Hopper JE, Workman JL. Gal80 confers specificity on HAT complex interactions with activators. J Biol Chem. 2002 Jul 5; 277(27):24648-52.
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11. Gribenko AV, Hopper JE, Makhatadze GI. Molecular characterization and tissue distribution of a novel member of the S100 family of EF-hand proteins. Biochemistry. 2001 Dec 25; 40(51):15538-48.
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12. Peng G, Hopper JE. Evidence for Gal3p's cytoplasmic location and Gal80p's dual cytoplasmic-nuclear location implicates new mechanisms for controlling Gal4p activity in Saccharomyces cerevisiae. Mol Cell Biol. 2000 Jul; 20(14):5140-8.
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13. Sil AK, Xin P, Hopper JE. Vectors allowing amplified expression of the Saccharomyces cerevisiae Gal3p-Gal80p-Gal4p transcription switch: applications to galactose-regulated high-level production of proteins. Protein Expr Purif. 2000 Mar; 18(2):202-12.
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14. Sil AK, Alam S, Xin P, Ma L, Morgan M, Lebo CM, Woods MP, Hopper JE. The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP. Mol Cell Biol. 1999 Nov; 19(11):7828-40.
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15. Blank TE, Woods MP, Lebo CM, Xin P, Hopper JE. Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae. Mol Cell Biol. 1997 May; 17(5):2566-75.
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16. Mylin LM, Hopper JE. Inducible expression cassettes in yeast: GAL4. Methods Mol Biol. 1997; 62:131-48.
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17. Long RM, Hopper JE. Genetic and carbon source regulation of phosphorylation of Sip1p, a Snf1p-associated protein involved in carbon response in Saccharomyces cerevisiae. Yeast. 1995 Mar; 11(3):233-46.
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18. Mylin LM, Bushman VL, Long RM, Yu X, Lebo CM, Blank TE, Hopper JE. SIP1 is a catabolite repression-specific negative regulator of GAL gene expression. Genetics. 1994 Jul; 137(3):689-700.
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19. Bhat PJ, Hopper JE. Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon. Mol Cell Biol. 1992 Jun; 12(6):2701-7.
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20. Mylin LM, Gerardot CJ, Hopper JE, Dickson RC. Sequence conservation in the Saccharomyces and Kluveromyces GAL11 transcription activators suggests functional domains. Nucleic Acids Res. 1991 Oct 11; 19(19):5345-50.
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21. Bhat PJ, Hopper JE. The mechanism of inducer formation in gal3 mutants of the yeast galactose system is independent of normal galactose metabolism and mitochondrial respiratory function. Genetics. 1991 Jun; 128(2):233-9.
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22. Long RM, Mylin LM, Hopper JE. GAL11 (SPT13), a transcriptional regulator of diverse yeast genes, affects the phosphorylation state of GAL4, a highly specific transcriptional activator. Mol Cell Biol. 1991 Apr; 11(4):2311-4.
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23. Mylin LM, Johnston M, Hopper JE. Phosphorylated forms of GAL4 are correlated with ability to activate transcription. Mol Cell Biol. 1990 Sep; 10(9):4623-9.
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24. Bhat PJ, Oh D, Hopper JE. Analysis of the GAL3 signal transduction pathway activating GAL4 protein-dependent transcription in Saccharomyces cerevisiae. Genetics. 1990 Jun; 125(2):281-91.
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25. Oh D, Hopper JE. Transcription of a yeast phosphoglucomutase isozyme gene is galactose inducible and glucose repressible. Mol Cell Biol. 1990 Apr; 10(4):1415-22.
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26. Mylin LM, Hofmann KJ, Schultz LD, Hopper JE. Regulated GAL4 expression cassette providing controllable and high-level output from high-copy galactose promoters in yeast. Methods Enzymol. 1990; 185:297-308.
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27. Mylin LM, Bhat JP, Hopper JE. Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. Genes Dev. 1989 Aug; 3(8):1157-65.
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28. Bajwa W, Torchia TE, Hopper JE. Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases. Mol Cell Biol. 1988 Aug; 8(8):3439-47.
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29. Baker SM, Johnston SA, Hopper JE, Jaehning JA. Transcription of multiple copies of the yeast GAL7 gene is limited by specific factors in addition to GAL4. Mol Gen Genet. 1987 Jun; 208(1-2):127-34.
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30. Riley MI, Hopper JE, Johnston SA, Dickson RC. GAL4 of Saccharomyces cerevisiae activates the lactose-galactose regulon of Kluyveromyces lactis and creates a new phenotype: glucose repression of the regulon. Mol Cell Biol. 1987 Feb; 7(2):780-6.
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31. Schultz LD, Hofmann KJ, Mylin LM, Montgomery DL, Ellis RW, Hopper JE. Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast. Gene. 1987; 61(2):123-33.
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32. Johnston SA, Zavortink MJ, Debouck C, Hopper JE. Functional domains of the yeast regulatory protein GAL4. Proc Natl Acad Sci U S A. 1986 Sep; 83(17):6553-7.
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33. Torchia TE, Hopper JE. Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae. Genetics. 1986 Jun; 113(2):229-46.
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34. Lohr D, Hopper JE. The relationship of regulatory proteins and DNase I hypersensitive sites in the yeast GAL1-10 genes. Nucleic Acids Res. 1985 Dec 9; 13(23):8409-23.
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35. Sumner-Smith M, Bozzato RP, Skipper N, Davies RW, Hopper JE. Analysis of the inducible MEL1 gene of Saccharomyces carlsbergensis and its secreted product, alpha-galactosidase (melibiase). Gene. 1985; 36(3):333-40.
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36. Torchia TE, Hamilton RW, Cano CL, Hopper JE. Disruption of regulatory gene GAL80 in Saccharomyces cerevisiae: effects on carbon-controlled regulation of the galactose/melibiose pathway genes. Mol Cell Biol. 1984 Aug; 4(8):1521-7.
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37. Post-Beittenmiller MA, Hamilton RW, Hopper JE. Regulation of basal and induced levels of the MEL1 transcript in Saccharomyces cerevisiae. Mol Cell Biol. 1984 Jul; 4(7):1238-45.
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38. Yarger JG, Halvorson HO, Hopper JE. Regulation of galactokinase (GAL1) enzyme accumulation in Saccharomyces cerevisiae. Mol Cell Biochem. 1984; 61(2):173-82.
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39. Johnston SA, Hopper JE. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon. Proc Natl Acad Sci U S A. 1982 Nov; 79(22):6971-5.
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40. Perlman D, Hopper JE. Constitutive synthesis of the GAL4 protein, a galactose pathway regulator in Saccharomyces cerevisiae. Cell. 1979 Jan; 16(1):89-95.
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41. Hopper JE, Rowe LB. Molecular expression and regulation of the galactose pathway genes in Saccharomyces cerevisiae. Distinct messenger RNAs specified by the Gali and Gal7 genes in the Gal7-Gal10-Gal1 cluster. J Biol Chem. 1978 Oct 25; 253(20):7566-9.
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42. Hopper JE, Broach JR, Rowe LB. Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function. Proc Natl Acad Sci U S A. 1978 Jun; 75(6):2878-82.
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43. Gallis BM, McDonnell JP, Hopper JE, Young ET. Translation of poly(riboadenylic acid)-enriched messenger RNAs from the yeast, Saccharomyces cerevisiae, in heterologous cell-free systems. Biochemistry. 1975 Mar 11; 14(5):1038-46.
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Co-Authors  
Bewley, Maria
Eckert, Kristin
Ehrlich, H
Ropson, Ira
Verderame, Michael
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