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Victor Canfield
Title Assistant Professor
Institution College of Medicine
Department Pharmacology
Address 500 University Drive Hershey PA 17033
Mailbox: R130
Telephone 7175314563
Email
Background
PREFERRED TITLE/ROLE:

Assistant Professor of Pharmacology

EDUCATION:

Ph.D., University of California, Berkeley, 1985
Postdoctoral Training, Roche Institute of Molecular Biology and Yale University, 1986-1992

NARRATIVE:

Current research interests include mechanisms involved in melanin pigmentation and the genetics of variations among human populations. Approaches employed include heterologous expression systems as well as bioinformatic analysis of genomic data.
Publications
1. Boehmler W, Petko J, Canfield VA, Levenson R. Genomic strategies for the identification of dopamine receptor genes in zebrafish. Methods Mol Biol. 2013; 964:201-14.
  View in: PubMed
 
2. Tsetskhladze ZR, Canfield VA, Ang KC, Wentzel SM, Reid KP, Berg AS, Johnson SL, Kawakami K, Cheng KC. Functional assessment of human coding mutations affecting skin pigmentation using zebrafish. PLoS One. 2012; 7(10):e47398.
  View in: PubMed
 
3. Ang KC, Ngu MS, Reid KP, Teh MS, Aida ZS, Koh DX, Berg A, Oppenheimer S, Salleh H, Clyde MM, Md-Zain BM, Canfield VA, Cheng KC. Skin color variation in Orang Asli tribes of Peninsular Malaysia. PLoS One. 2012; 7(8):e42752.
  View in: PubMed
 
4. Petko JA, Kabbani N, Frey C, Woll M, Hickey K, Craig M, Canfield VA, Levenson R. Proteomic and functional analysis of NCS-1 binding proteins reveals novel signaling pathways required for inner ear development in zebrafish. BMC Neurosci. 2009; 10:27.
  View in: PubMed
 
5. Boehmler W, Petko J, Woll M, Frey C, Thisse B, Thisse C, Canfield VA, Levenson R. Identification of zebrafish A2 adenosine receptors and expression in developing embryos. Gene Expr Patterns. 2009 Mar; 9(3):144-51.
  View in: PubMed
 
6. Petko JA, Millimaki BB, Canfield VA, Riley BB, Levenson R. Otoc1: a novel otoconin-90 ortholog required for otolith mineralization in zebrafish. Dev Neurobiol. 2008 Feb 1; 68(2):209-22.
  View in: PubMed
 
7. Boehmler W, Carr T, Thisse C, Thisse B, Canfield VA, Levenson R. D4 Dopamine receptor genes of zebrafish and effects of the antipsychotic clozapine on larval swimming behaviour. Genes Brain Behav. 2007 Mar; 6(2):155-66.
  View in: PubMed
 
8. Norton HL, Kittles RA, Parra E, McKeigue P, Mao X, Cheng K, Canfield VA, Bradley DG, McEvoy B, Shriver MD. Genetic evidence for the convergent evolution of light skin in Europeans and East Asians. Mol Biol Evol. 2007 Mar; 24(3):710-22.
  View in: PubMed
 
9. Chi A, Valencia JC, Hu ZZ, Watabe H, Yamaguchi H, Mangini NJ, Huang H, Canfield VA, Cheng KC, Yang F, Abe R, Yamagishi S, Shabanowitz J, Hearing VJ, Wu C, Appella E, Hunt DF. Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J Proteome Res. 2006 Nov; 5(11):3135-44.
  View in: PubMed
 
10. Blasiole B, Canfield VA, Vollrath MA, Huss D, Mohideen MA, Dickman JD, Cheng KC, Fekete DM, Levenson R. Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish. Dev Biol. 2006 Jun 1; 294(1):148-60.
  View in: PubMed
 
11. Lamason RL, Mohideen MA, Mest JR, Wong AC, Norton HL, Aros MC, Jurynec MJ, Mao X, Humphreville VR, Humbert JE, Sinha S, Moore JL, Jagadeeswaran P, Zhao W, Ning G, Makalowska I, McKeigue PM, O'donnell D, Kittles R, Parra EJ, Mangini NJ, Grunwald DJ, Shriver MD, Canfield VA, Cheng KC. SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans. Science. 2005 Dec 16; 310(5755):1782-6.
  View in: PubMed
 
12. Croushore JA, Blasiole B, Riddle RC, Thisse C, Thisse B, Canfield VA, Robertson GP, Cheng KC, Levenson R. Ptena and ptenb genes play distinct roles in zebrafish embryogenesis. Dev Dyn. 2005 Dec; 234(4):911-21.
  View in: PubMed
 
13. Blasiole B, Kabbani N, Boehmler W, Thisse B, Thisse C, Canfield V, Levenson R. Neuronal calcium sensor-1 gene ncs-1a is essential for semicircular canal formation in zebrafish inner ear. J Neurobiol. 2005 Sep 5; 64(3):285-97.
  View in: PubMed
 
14. Boehmler W, Obrecht-Pflumio S, Canfield V, Thisse C, Thisse B, Levenson R. Evolution and expression of D2 and D3 dopamine receptor genes in zebrafish. Dev Dyn. 2004 Jul; 230(3):481-93.
  View in: PubMed
 
15. Blasiole B, Degrave A, Canfield V, Boehmler W, Thisse C, Thisse B, Mohideen MA, Levenson R. Differential expression of Na,K-ATPase alpha and beta subunit genes in the developing zebrafish inner ear. Dev Dyn. 2003 Nov; 228(3):386-92.
  View in: PubMed
 
16. Lin R, Canfield V, Levenson R. Dominant negative mutants of filamin A block cell surface expression of the D2 dopamine receptor. Pharmacology. 2002 Dec; 66(4):173-81.
  View in: PubMed
 
17. Blasiole B, Canfield V, Degrave A, Thisse C, Thisse B, Rajarao J, Levenson R. Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase alpha1 subunit gene (atp1a1a.5). Gene Expr Patterns. 2002 Dec; 2(3-4):243-6.
  View in: PubMed
 
18. Blasiole B, Canfield V, Degrave A, Thisse C, Thisse B, Rajarao J, Levenson R. Cloning, mapping, and developmental expression of a sixth zebrafish Na,K-ATPase alpha1 subunit gene (atp1a1a.5). Mech Dev. 2002 Dec; 119 Suppl 1:S211-4.
  View in: PubMed
 
19. Canfield VA, Loppin B, Thisse B, Thisse C, Postlethwait JH, Mohideen MA, Rajarao SJ, Levenson R. Na,K-ATPase alpha and beta subunit genes exhibit unique expression patterns during zebrafish embryogenesis. Mech Dev. 2002 Aug; 116(1-2):51-9.
  View in: PubMed
 
20. Rajarao JR, Canfield VA, Loppin B, Thisse B, Thisse C, Yan YL, Postlethwait JH, Levenson R. Two Na,K-ATPase beta 2 subunit isoforms are differentially expressed within the central nervous system and sensory organs during zebrafish embryogenesis. Dev Dyn. 2002 Mar; 223(2):254-61.
  View in: PubMed
 
21. Sweeney G, Niu W, Canfield VA, Levenson R, Klip A. Insulin increases plasma membrane content and reduces phosphorylation of Na(+)-K(+) pump alpha(1)-subunit in HEK-293 cells. Am J Physiol Cell Physiol. 2001 Dec; 281(6):C1797-803.
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22. Rajarao SJ, Canfield VA, Mohideen MA, Yan YL, Postlethwait JH, Cheng KC, Levenson R. The repertoire of Na,K-ATPase alpha and beta subunit genes expressed in the zebrafish, Danio rerio. Genome Res. 2001 Jul; 11(7):1211-20.
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23. Green N, Fang H, Kalies KU, Canfield V. Determining the topology of an integral membrane protein. Curr Protoc Cell Biol. 2001 May; Chapter 5:Unit 5.2.
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24. Dahl JP, Binda A, Canfield VA, Levenson R. Participation of Na,K-ATPase in FGF-2 secretion: rescue of ouabain-inhibitable FGF-2 secretion by ouabain-resistant Na,K-ATPase alpha subunits. Biochemistry. 2000 Dec 5; 39(48):14877-83.
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25. Underhill DA, Canfield VA, Dahl JP, Gros P, Levenson R. The Na,K-ATPase alpha4 gene (Atp1a4) encodes a ouabain-resistant alpha subunit and is tightly linked to the alpha2 gene (Atp1a2) on mouse chromosome 1. Biochemistry. 1999 Nov 9; 38(45):14746-51.
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26. Canfield VA, Levenson R. Domain swapping between Na,K- and H,K-ATPase identifies regions that specify Na,K-ATPase activity. Biochemistry. 1998 May 19; 37(20):7509-16.
  View in: PubMed
 
27. Malik N, Canfield V, Sanchez-Watts G, Watts AG, Scherer S, Beatty BG, Gros P, Levenson R. Structural organization and chromosomal localization of the human Na,K-ATPase beta 3 subunit gene and pseudogene. Mamm Genome. 1998 Feb; 9(2):136-43.
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28. Canfield VA, Norbeck L, Levenson R. Localization of cytoplasmic and extracellular domains of Na,K-ATPase by epitope tag insertion. Biochemistry. 1996 Nov 12; 35(45):14165-72.
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29. Malik N, Canfield VA, Beckers MC, Gros P, Levenson R. Identification of the mammalian Na,K-ATPase 3 subunit. J Biol Chem. 1996 Sep 13; 271(37):22754-8.
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30. Kast C, Canfield V, Levenson R, Gros P. Transmembrane organization of mouse P-glycoprotein determined by epitope insertion and immunofluorescence. J Biol Chem. 1996 Apr 19; 271(16):9240-8.
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31. Canfield V, West AB, Goldenring JR, Levenson R. Genetic ablation of parietal cells in transgenic mice: a new model for analyzing cell lineage relationships in the gastric mucosa. Proc Natl Acad Sci U S A. 1996 Mar 19; 93(6):2431-5.
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32. Kast C, Canfield V, Levenson R, Gros P. Membrane topology of P-glycoprotein as determined by epitope insertion: transmembrane organization of the N-terminal domain of mdr3. Biochemistry. 1995 Apr 4; 34(13):4402-11.
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33. Canfield VA, Levenson R. Transmembrane organization of the Na,K-ATPase determined by epitope addition. Biochemistry. 1993 Dec 21; 32(50):13782-6.
  View in: PubMed
 
34. Malo D, Gros P, Bergmann A, Trask B, Mohrenweiser HW, Canfield VA, Levenson R. Genes encoding the H,K-ATPase alpha and Na,K-ATPase alpha 3 subunits are linked on mouse chromosome 7 and human chromosome 19. Mamm Genome. 1993 Nov; 4(11):644-9.
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35. Canfield VA, Xu KY, D'Aquila T, Shyjan AW, Levenson R. Molecular cloning and characterization of Na,K-ATPase from Hydra vulgaris: implications for enzyme evolution and ouabain sensitivity. New Biol. 1992 Apr; 4(4):339-48.
  View in: PubMed
 
36. Shyjan AW, Canfield VA, Levenson R. Evolution of the Na,K- and H,K-ATPase beta subunit gene family: structure of the murine Na,K-ATPase beta 2 subunit gene. Genomics. 1991 Oct; 11(2):435-42.
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37. Canfield VA, Levenson R. Structural organization and transcription of the mouse gastric H+, K(+)-ATPase beta subunit gene. Proc Natl Acad Sci U S A. 1991 Sep 15; 88(18):8247-51.
  View in: PubMed
 
38. Malo D, Schurr E, Dorfman J, Canfield V, Levenson R, Gros P. Three brain sodium channel alpha-subunit genes are clustered on the proximal segment of mouse chromosome 2. Genomics. 1991 Jul; 10(3):666-72.
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39. Canfield VA, Okamato CT, Chow D, Dorfman J, Gros P, Forte JG, Levenson R. Identification and characterization of a gene for the H,K-ATPase beta-subunit. Soc Gen Physiol Ser. 1991; 46:89-98.
  View in: PubMed
 
40. Canfield VA, Okamoto CT, Chow D, Dorfman J, Gros P, Forte JG, Levenson R. Cloning of the H,K-ATPase beta subunit. Tissue-specific expression, chromosomal assignment, and relationship to Na,K-ATPase beta subunits. J Biol Chem. 1990 Nov 15; 265(32):19878-84.
  View in: PubMed
 
41. Canfield V, Emanuel JR, Spickofsky N, Levenson R, Margolskee RF. Ouabain-resistant mutants of the rat Na,K-ATPase alpha 2 isoform identified by using an episomal expression vector. Mol Cell Biol. 1990 Apr; 10(4):1367-72.
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Co-Authors  
Berg, Arthur
Cheng, Keith
Levenson, Robert
Robertson, Gavin
Shriver, Mark
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