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Samuel Zhang
Title Assistant Professor
Institution College of Medicine
Department Neural and Behavioral Sciences
Division Neural & Behavioral Science
Address 500 University Drive Hershey PA 17033
Mailbox: H109
Telephone 7175318480
Email
Background
PREFERRED TITLE/ROLE:

Assistant Professor of Neural & Behavioral Sciences

GRADUATE PROGRAM AFFILIATIONS:

Anatomy, Biomedical Sciences (BMS), Neuroscience

EDUCATION:

M.D., Henan Medical College, China
Ph.D., University of Tokyo, Japan
Post-doctoral, Yale School of Medicine, New Haven

NARRATIVE:

Signal Transduction in Mammal Development and Pathogenesis
The focus of Zhang's lab is to understand the molecular basis of signal transducer and activator of transcription (STAT) proteins during mammalian development and pathogenesis. STAT were originally identified and isolated using cell culture systems that respond to cytokines such as interferon. It is well established that the STAT proteins have a range of essential functions for generation and function of the immune system. It is also widely accepted that STAT proteins co-evolved with the innate and adaptive immunity that occurred in the transition from lower to higher organisms. Tissue specific STAT3 disruption causes several phenotypes related to human diseases such as Crohn's disease-like pathogenesis, heart failure, and severe inflammation. Growing experimental evidences that STAT pathways are also involved in many other aspects of vertebrate development. It has been found that several STAT proteins are active during early mammalian development. Recently we show that STAT3 mediated signaling is crucial for the inhibitory function of CNTF during rod photoreceptor development. Activation of STAT3 keeps the undifferentiation status of postnatal precursors in retina. STAT3 is also critical for retina Muller glial reactivation in response to stresses. These indicated that STAT as common factors have specific roles on specific cell types. To understand their tissue specific functions, we use retina as a model to study STAT function in neuron and glia cells.

Systems Biology of Neural Development and Functions
The retina is a well-defined portion of the central nervous system (CNS) that has long been used as a model for CNS developmental and functional studies. It is susceptible to a variety of diseases that can lead to vision loss or complete blindness. Most of the unique functions of the retina depend upon its tissue-specific transcripts, suggesting that a systematic definition of retinal transcripts would be an invaluable approach to understanding retinal cell identities and functions. Traditional method allows functional relationship only among few genes, the development of microarray techniques changes the way to understand a biological process as a whole among the major genes played. We have generated a comprehensive mouse retina transcriptome based on 81,000 murine retina transcripts from whole mouse ESTs. About 33,000 sequence-unique retina transcript clusters (RTCs) have been identified and the highest-grade retina-enriched pool covered almost all the known genes in phototransduction processes that involved in human retina diseases, suggesting the potential of gene discoveries for human retina disorders. We have also generated mouse retina specific cDNA microarray that represented about 10,000 UniGene clusters for studying the biological process during retina development or downstream network alternatives by gene targeting in mouse retina. Integrated biological and computational methods, our goal is to understand the gene regulation network during retina development and pathogenesis.
Publications
1. Popova EY, Grigoryev SA, Fan Y, Skoultchi AI, Zhang SS, Barnstable CJ. Developmentally Regulated Linker Histone H1c Promotes Heterochromatin Condensation and Mediates Structural Integrity of Rod Photoreceptors in Mouse Retina. J Biol Chem. 2013 May 3.
  View in: PubMed
 
2. Zhang S, Li W, Wang W, Zhang SS, Huang P, Zhang C. Expression and Activation of STAT3 in the Astrocytes of Optic Nerve in a Rat Model of Transient Intraocular Hypertension. PLoS One. 2013; 8(1):e55683.
  View in: PubMed
 
3. Popova EY, Xu X, Dewan AT, Salzberg AC, Berg A, Hoh J, Zhang SS, Barnstable CJ. Stage and Gene Specific Signatures Defined by Histones H3K4me2 and H3K27me3 Accompany Mammalian Retina Maturation In Vivo. PLoS One. 2012; 7(10):e46867.
  View in: PubMed
 
4. Pechkovsky DV, Prêle CM, Wong J, Hogaboam CM, McAnulty RJ, Laurent GJ, Zhang SS, Selman M, Mutsaers SE, Knight DA. STAT3-mediated signaling dysregulates lung fibroblast-myofibroblast activation and differentiation in UIP/IPF. Am J Pathol. 2012 Apr; 180(4):1398-412.
  View in: PubMed
 
5. Avella DM, Li G, Schell TD, Liu D, Zhang SS, Lou X, Berg A, Kimchi ET, Tagaram HR, Yang Q, Shereef S, Garcia LS, Kester M, Isom HC, Rountree CB, Staveley-O'Carroll KF. Regression of established hepatocellular carcinoma is induced by chemoimmunotherapy in an orthotopic murine model. Hepatology. 2012 Jan; 55(1):141-52.
  View in: PubMed
 
6. Pinzon-Guzman C, Zhang SS, Barnstable CJ. Specific protein kinase C isoforms are required for rod photoreceptor differentiation. J Neurosci. 2011 Dec 14; 31(50):18606-17.
  View in: PubMed
 
7. Huang P, Shi Y, Wang X, Zhang SS, Zhang C. Use of microperimetry to compare macular light sensitivity in eyes with open-angle and angle-closure glaucoma. Jpn J Ophthalmol. 2012 Mar; 56(2):138-44.
  View in: PubMed
 
8. Zhang SS, Li H, Huang P, Lou LX, Fu XY, Barnstable CJ. MAPK signaling during Müller glial cell development in retina explant cultures. J Ocul Biol Dis Infor. 2010 Dec; 3(4):129-33.
  View in: PubMed
 
9. Pan YL, Berg MJ, Zhang SS, Noh H, Cao H, Chang RK, Videen G. Measurement and autocorrelation analysis of two-dimensional light-scattering patterns from living cells for label-free classification. Cytometry A. 2011 Apr; 79(4):284-92.
  View in: PubMed
 
10. Huang P, Xu Y, Wei R, Li H, Tang Y, Liu J, Zhang SS, Zhang C. Efficacy of tetrandrine on lowering intraocular pressure in animal model with ocular hypertension. J Glaucoma. 2011 Mar; 20(3):183-8.
  View in: PubMed
 
11. Huang P, Zhang C, Wang W, Zhang SM, Barnstable CJ. [Purified mouse retinal ganglion cell culture]. Zhonghua Yan Ke Za Zhi. 2011 Feb; 47(2):129-33.
  View in: PubMed
 
12. Liu X, Yang JM, Zhang SS, Liu XY, Liu DX. Induction of cell cycle arrest at G1 and S phases and cAMP-dependent differentiation in C6 glioma by low concentration of cycloheximide. BMC Cancer. 2010; 10:684.
  View in: PubMed
 
13. Huang P, Qi Y, Xu YS, Liu J, Liao D, Zhang SS, Zhang C. Serum cytokine alteration is associated with optic neuropathy in human primary open angle glaucoma. J Glaucoma. 2010 Jun-Jul; 19(5):324-30.
  View in: PubMed
 
14. Qi S, Wang Y, Zhou M, Ge Y, Yan Y, Wang J, Zhang SS, Zhang S. A mitochondria-localized glutamic acid-rich protein (MGARP/OSAP) is highly expressed in retina that exhibits a large area of intrinsic disorder. Mol Biol Rep. 2011 Jun; 38(5):2869-77.
  View in: PubMed
 
15. Pinzon-Guzman C, Shaomin Zhang S, Barnstable CJ. Protein kinase C regulates rod photoreceptor differentiation through modulation of STAT3 signaling. Adv Exp Med Biol. 2010; 664:21-8.
  View in: PubMed
 
16. Huang P, Zhang SS, Zhang C. Erratum: The two sides of cytokine signaling and glaucomatous optic neuropathy. J Ocul Biol Dis Infor. 2009 Sep; 2(3):98-103.
  View in: PubMed
 
17. Huang P, Zhang SS, Zhang C. The two sides of cytokine signaling and glaucomatous optic neuropathy. J Ocul Biol Dis Infor. 2009 Jun; 2(2):78-83.
  View in: PubMed
 
18. Liu MG, Li H, Xu X, Barnstable CJ, Zhang SS. Comparison of gene expression during in vivo and in vitro postnatal retina development. J Ocul Biol Dis Infor. 2008 Dec; 1(2-4):59-72.
  View in: PubMed
 
19. Zhang C, Li H, Liu MG, Kawasaki A, Fu XY, Barnstable CJ, Shao-Min Zhang S. STAT3 activation protects retinal ganglion cell layer neurons in response to stress. Exp Eye Res. 2008 Jun; 86(6):991-7.
  View in: PubMed
 
20. Moh A, Iwamoto Y, Chai GX, Zhang SS, Kano A, Yang DD, Zhang W, Wang J, Jacoby JJ, Gao B, Flavell RA, Fu XY. Role of STAT3 in liver regeneration: survival, DNA synthesis, inflammatory reaction and liver mass recovery. Lab Invest. 2007 Oct; 87(10):1018-28.
  View in: PubMed
 
21. Dewan A, Liu M, Hartman S, Zhang SS, Liu DT, Zhao C, Tam PO, Chan WM, Lam DS, Snyder M, Barnstable C, Pang CP, Hoh J. HTRA1 promoter polymorphism in wet age-related macular degeneration. Science. 2006 Nov 10; 314(5801):989-92.
  View in: PubMed
 
22. Zhang SS, Xu X, Liu MG, Zhao H, Soares MB, Barnstable CJ, Fu XY. A biphasic pattern of gene expression during mouse retina development. BMC Dev Biol. 2006; 6:48.
  View in: PubMed
 
23. Xu X, Zhang SS, Barnstable CJ, Tombran-Tink J. Molecular phylogeny of the antiangiogenic and neurotrophic serpin, pigment epithelium derived factor in vertebrates. BMC Genomics. 2006; 7:248.
  View in: PubMed
 
24. Zhang X, Shan P, Jiang G, Zhang SS, Otterbein LE, Fu XY, Lee PJ. Endothelial STAT3 is essential for the protective effects of HO-1 in oxidant-induced lung injury. FASEB J. 2006 Oct; 20(12):2156-8.
  View in: PubMed
 
25. Chen L, Zhang SS, Barnstable CJ, Tombran-Tink J. PEDF induces apoptosis in human endothelial cells by activating p38 MAP kinase dependent cleavage of multiple caspases. Biochem Biophys Res Commun. 2006 Oct 6; 348(4):1288-95.
  View in: PubMed
 
26. Tombran-Tink J, Aparicio S, Xu X, Tink AR, Lara N, Sawant S, Barnstable CJ, Zhang SS. PEDF and the serpins: phylogeny, sequence conservation, and functional domains. J Struct Biol. 2005 Aug; 151(2):130-50.
  View in: PubMed
 
27. Zhang SS, Liu MG, Kano A, Zhang C, Fu XY, Barnstable CJ. STAT3 activation in response to growth factors or cytokines participates in retina precursor proliferation. Exp Eye Res. 2005 Jul; 81(1):103-15.
  View in: PubMed
 
28. Zhang SS, Xu X, Li J, Liu MG, Zhao H, Soares MB, Barnstable CJ, Fu XY. Comprehensive in silico functional specification of mouse retina transcripts. BMC Genomics. 2005; 6:40.
  View in: PubMed
 
29. Zhang SS, Wei J, Qin H, Zhang L, Xie B, Hui P, Deisseroth A, Barnstable CJ, Fu XY. STAT3-mediated signaling in the determination of rod photoreceptor cell fate in mouse retina. Invest Ophthalmol Vis Sci. 2004 Jul; 45(7):2407-12.
  View in: PubMed
 
30. Ishikawa T, Zhang SS, Qin X, Takahashi Y, Oda H, Nakatsuru Y, Ide F. DNA repair and cancer: lessons from mutant mouse models. Cancer Sci. 2004 Feb; 95(2):112-7.
  View in: PubMed
 
31. Pan, Y., Boutou, V., Bottiger, JR., Zhang, SS., Wolf, J., and Chang, RK. Aerosol Science and Technology. A puff of air sorts bioaerosols for pathogen identification. 2004; (38):598-602.
 
32. Xiao C, Shim JH, Klüppel M, Zhang SS, Dong C, Flavell RA, Fu XY, Wrana JL, Hogan BL, Ghosh S. Ecsit is required for Bmp signaling and mesoderm formation during mouse embryogenesis. Genes Dev. 2003 Dec 1; 17(23):2933-49.
  View in: PubMed
 
33. Jacoby JJ, Kalinowski A, Liu MG, Zhang SS, Gao Q, Chai GX, Ji L, Iwamoto Y, Li E, Schneider M, Russell KS, Fu XY. Cardiomyocyte-restricted knockout of STAT3 results in higher sensitivity to inflammation, cardiac fibrosis, and heart failure with advanced age. Proc Natl Acad Sci U S A. 2003 Oct 28; 100(22):12929-34.
  View in: PubMed
 
34. Li M, Zhang SS, Barnstable CJ. Developmental and tissue expression patterns of mouse Mpp4 gene. Biochem Biophys Res Commun. 2003 Jul 25; 307(2):229-35.
  View in: PubMed
 
35. Zhang SS, Wei JY, Li C, Barnstable CJ, Fu XY. Expression and activation of STAT proteins during mouse retina development. Exp Eye Res. 2003 Apr; 76(4):421-31.
  View in: PubMed
 
36. Welte T, Zhang SS, Wang T, Zhang Z, Hesslein DG, Yin Z, Kano A, Iwamoto Y, Li E, Craft JE, Bothwell AL, Fikrig E, Koni PA, Flavell RA, Fu XY. STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality: a critical role of STAT3 in innate immunity. Proc Natl Acad Sci U S A. 2003 Feb 18; 100(4):1879-84.
  View in: PubMed
 
37. Zhang SS, Fu XY, Barnstable CJ. Tissue culture studies of retinal development. Methods. 2002 Dec; 28(4):439-47.
  View in: PubMed
 
38. Zhang SS, Fu XY, Barnstable CJ. Molecular aspects of vertebrate retinal development. Mol Neurobiol. 2002 Oct-Dec; 26(2-3):137-52.
  View in: PubMed
 
39. Takahashi Y, Nakatsuru Y, Zhang S, Shimizu Y, Kume H, Tanaka K, Ide F, Ishikawa T. Enhanced spontaneous and aflatoxin-induced liver tumorigenesis in xeroderma pigmentosum group A gene-deficient mice. Carcinogenesis. 2002 Apr; 23(4):627-33.
  View in: PubMed
 
40. Ishikawa T, Ide F, Qin X, Zhang S, Takahashi Y, Sekiguchi M, Tanaka K, Nakatsuru Y. Importance of DNA repair in carcinogenesis: evidence from transgenic and gene targeting studies. Mutat Res. 2001 Jun 2; 477(1-2):41-9.
  View in: PubMed
 
41. Zhang SS, Welte T, Fu XY. Dysfunction of Stat4 leads to accelerated incidence of chemical-induced thymic lymphomas in mice. Exp Mol Pathol. 2001 Jun; 70(3):231-8.
  View in: PubMed
 
42. Qin X, Zhang S, Matsukuma S, Zarkovic M, Shimizu S, Ishikawa T, Nakatsuru Y. Protection against malignant progression of spontaneously developing liver tumors in transgenic mice expressing O(6)-methylguanine-DNA methyltransferase. Jpn J Cancer Res. 2000 Nov; 91(11):1085-9.
  View in: PubMed
 
43. Tsurutani N, Oda H, Nakatsuru Y, Imai Y, Zhang S, Ueno Y, Ishikawa T. cDNA cloning and developmental expression of the porcine homologue of WT1. Gene. 1998 May 12; 211(2):215-20.
  View in: PubMed
 
44. Oda H, Zhang S, Tsurutani N, Shimizu S, Nakatsuru Y, Aizawa S, Ishikawa T. Loss of p53 is an early event in induction of brain tumors in mice by transplacental carcinogen exposure. Cancer Res. 1997 Feb 15; 57(4):646-50.
  View in: PubMed
 
45. Qin X, Zhang S, Zarkovic M, Nakatsuru Y, Shimizu S, Yamazaki Y, Oda H, Nikaido O, Ishikawa T. Detection of ultraviolet photoproducts in mouse skin exposed to natural sunlight. Jpn J Cancer Res. 1996 Jul; 87(7):685-90.
  View in: PubMed
 
46. Tsuzuki T, Sakumi K, Shiraishi A, Kawate H, Igarashi H, Iwakuma T, Tominaga Y, Zhang S, Shimizu S, Ishikawa T et AL. Targeted disruption of the DNA repair methyltransferase gene renders mice hypersensitive to alkylating agent. Carcinogenesis. 1996 Jun; 17(6):1215-20.
  View in: PubMed
 
47. Qin X, Zhang S, Oda H, Nakatsuru Y, Shimizu S, Yamazaki Y, Nikaido O, Ishikawa T. Quantitative detection of ultraviolet light-induced photoproducts in mouse skin by immunohistochemistry. Jpn J Cancer Res. 1995 Nov; 86(11):1041-8.
  View in: PubMed
 
48. Qin X, Zhang S, Zarkovic M, Yamazaki Y, Oda H, Nakatsuru Y, Ishikawa T, Ishikawa T. Inhibitory effect of probucol on nephrotoxicity induced by ferric nitrilotriacetate (Fe-NTA) in rats. Carcinogenesis. 1995 Oct; 16(10):2549-52.
  View in: PubMed
 
49. Zarkovic M, Qin X, Nakatsuru Y, Zhang S, Yamazaki Y, Oda H, Ishikawa T, Ishikawa T. Inhibitory effect of probucol on benzo[a]pyrene induced lung tumorigenesis. Carcinogenesis. 1995 Oct; 16(10):2599-601.
  View in: PubMed
 
50. Qin X, Zhang S, Nakatsuru Y, Oda H, Yamazaki Y, Suzuki T, Nikaido O, Ishikawa T. Detection of active UV-photoproduct repair in monkey skin in vivo by quantitative immunohistochemistry. Cancer Lett. 1994 Aug 15; 83(1-2):291-8.
  View in: PubMed
 
 
Keyword
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Co-Authors  
Barnstable, Colin
Berg, Arthur
Kester, Mark
Staveley-O'Carroll, Kevin
Tombran-Tink, Joyce
See all (12) people
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