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Stephen Rannels
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Title Associate Professor Emeritus
Institution College of Medicine
Department Cellular and Molecular Physiology
Address 500 University Drive Hershey PA 17033
 Mailbox: H166
Email
Background
PREFERRED TITLE/ROLE:

Associate Professor Emeritus of Cellular and Molecular Physiology, Graduate Student Coordinator

SECONDARY APPOINTMENT(S)/ INSTITUTE(S)/ CENTER(S):

The Huck Institutes of the Life Sciences

GRADUATE PROGRAM AFFILIATIONS:

Physiology, Cell and Molecular Biology

EDUCATION:

Ph.D. Degree, The Pennsylvania State University College of Medicine, 1977;
Postdoctoral Training, Vanderbilt University; University of Otago, New Zealand, 1978-1982

NARRATIVE:

Our research interests aim to define the physiological regulation and functional significance of vitamin K-dependent g-carboxylation of protein substrates by rat lung and alveolar type II cells. Functional differences in the lung carboxylase enzyme are being compared with the well-known hepatic system, where several of the clotting factors are carboxylated at specific glutamic acid residues. This research will define microsomal carboxylase activity and substrate specificity in freshly isolated type II epithelial cells from normal, hormonally and nutritionally manipulated adult rats. The role of type II cell differentiation in modulating g-carboxylase activity and substrate specificity will be determined during primary culture where differentiated morphology and function can be modified by both laminin- and fibronectin-rich extracellular matrices. Current research has identified one protein named matrix Gla protein (MGP) in type II cells. MGP mRNA and protein are induced during culture and can be regulated by vitamin K status and the extracellular matrix. The identification of additional substrates will lead to a better understanding of both type II and type I cell function as several carboxylated proteins are expressed only after type II cells change to a type I cell-like phenotype in primary culture.

Another area of research interest includes studies of fetal lung development using a rat model in which the intracellular substrate supply for surfactant phospholipids is impaired due to a deficiency of phosphorylase b kinase. Non-glycogen substrate supply is studied in whole lung and isolated fetal type II cells. The coordination of phospholipid and surfactant protein (SP-A) transcription and translation will be investigated, as a unique dissociation of these pathways using this strain has been observed. This model has additional potential to explore the reasons for lung immaturity in diabetes, as a common feature of this disease is delayed fetal lung glycogenolysis.
Publications
1. Gilbert KA, Rannels SR. Matrix GLA protein modulates branching morphogenesis in fetal rat lung. Am J Physiol Lung Cell Mol Physiol. 2004 Jun; 286(6):L1179-87.
  View in: PubMed
 
2. Gilbert KA, Rannels SR. Glucocorticoid effects on vitamin K-dependent carboxylase activity and matrix Gla protein expression in rat lung. Am J Physiol Lung Cell Mol Physiol. 2003 Sep; 285(3):L569-77.
  View in: PubMed
 
3. Brown LM, Welch DR, Rannels DE, Rannels SR. Partial pneumonectomy enhances melanoma metastasis to mouse lungs. Chest. 2002 Mar; 121(3 Suppl):28S-29S.
  View in: PubMed
 
4. Brown LM, Welch DR, Rannels SR. B16F10 melanoma cell colonization of mouse lung is enhanced by partial pneumonectomy. Clin Exp Metastasis. 2002; 19(5):369-76.
  View in: PubMed
 
5. Brown LM, Rannels SR, Rannels DE. Implications of post-pneumonectomy compensatory lung growth in pulmonary physiology and disease. Respir Res. 2001; 2(6):340-7.
  View in: PubMed
 
6. Brown LM, Malkinson AM, Rannels DE, Rannels SR. Compensatory lung growth after partial pneumonectomy enhances lung tumorigenesis induced by 3-methylcholanthrene. Cancer Res. 1999 Oct 15; 59(20):5089-92.
  View in: PubMed
 
7. Rannels SR, Liu L, Weaver TE. Expression of glycogen phosphorylase isozymes in developing rat lung. Am J Physiol. 1997 Aug; 273(2 Pt 1):L389-94.
  View in: PubMed
 
8. Brown LM, Fox HL, Hazen SA, LaNoue KF, Rannels SR, Lynch CJ. Role of the matrixin MMP-2 in multicellular organization of adipocytes cultured in basement membrane components. Am J Physiol. 1997 Mar; 272(3 Pt 1):C937-49.
  View in: PubMed
 
9. Rannels SR. Impaired surfactant synthesis in fetal type II lung cells from gsd/gsd rats. Exp Lung Res. 1996 Mar-Apr; 22(2):213-29.
  View in: PubMed
 
10. Dunsmore SE, Rannels SR, Grove RN, Rannels DE. Adrenal hormone regulation of extracellular matrix synthesis by type II cells. Am J Physiol. 1995 Jun; 268(6 Pt 1):L885-93.
  View in: PubMed
 
11. Rannels SR, Cancela ML, Wolpert EB, Price PA. Matrix Gla protein mRNA expression in cultured type II pneumocytes. Am J Physiol. 1993 Sep; 265(3 Pt 1):L270-8.
  View in: PubMed
 
12. Rannels SR, Rannels SL, Sneyd JG, Loten EG. Fetal lung development in rats with a glycogen storage disorder. Am J Physiol. 1991 Jun; 260(6 Pt 1):L419-27.
  View in: PubMed
 
13. Gallaher KJ, Wolpert EB, Rannels SR. Gamma-carboxyglutamic acid excretion into rat amniotic fluid during late gestation. J Dev Physiol. 1990 Jun; 13(6):327-32.
  View in: PubMed
 
14. Rannels DE, Kameji R, Pegg AE, Rannels SR. Spermidine uptake by type II pneumocytes: interactions of amine uptake pathways. Am J Physiol. 1989 Dec; 257(6 Pt 1):L346-53.
  View in: PubMed
 
15. Uhal BD, Rannels SR, Rannels DE. Flow cytometric identification and isolation of hypertrophic type II pneumocytes after partial pneumonectomy. Am J Physiol. 1989 Sep; 257(3 Pt 1):C528-36.
  View in: PubMed
 
16. Russo LA, Rannels SR, Laslow KS, Rannels DE. Stretch-related changes in lung cAMP after partial pneumonectomy. Am J Physiol. 1989 Aug; 257(2 Pt 1):E261-8.
  View in: PubMed
 
17. Rannels DE, Rannels SR. Influence of the extracellular matrix on type 2 cell differentiation. Chest. 1989 Jul; 96(1):165-73.
  View in: PubMed
 
18. Gallaher KJ, Rannels DE, Rannels SR. Vitamin K-dependent carboxylase activity in fetal rat lung: developmental effects of dexamethasone and triiodothyronine. Pediatr Res. 1989 May; 25(5):530-4.
  View in: PubMed
 
19. Rannels SR, Grove RN, Rannels DE. Matrix-derived soluble components influence type II pneumocytes in primary culture. Am J Physiol. 1989 Mar; 256(3 Pt 1):C621-9.
  View in: PubMed
 
20. Rannels SR, Rannels DE. The type II pneumocyte as a model of lung cell interaction with the extracellular matrix. J Mol Cell Cardiol. 1989 Feb; 21 Suppl 1:151-9.
  View in: PubMed
 
21. Kameji R, Rannels SR, Pegg AE, Rannels DE. Spermidine uptake by type II pulmonary epithelial cells in primary culture. Am J Physiol. 1989 Jan; 256(1 Pt 1):C160-7.
  View in: PubMed
 
22. Rannels SR, Rannels DE. Alterations in type II pneumocytes cultured after partial pneumonectomy. Am J Physiol. 1988 May; 254(5 Pt 1):C684-90.
  View in: PubMed
 
23. Wallin R, Rannels SR. Identification of vitamin K-dependent carboxylase activity in lung type II cells but not in lung macrophages. Biochem J. 1988 Mar 1; 250(2):557-63.
  View in: PubMed
 
24. Rannels DE, Rannels SR. Compensatory growth of the lung following partial pneumonectomy. Exp Lung Res. 1988; 14(2):157-82.
  View in: PubMed
 
25. Rannels SR, Fisher CS, Heuser LJ, Rannels DE. Culture of type II pneumocytes on a type II cell-derived fibronectin-rich matrix. Am J Physiol. 1987 Dec; 253(6 Pt 1):C759-65.
  View in: PubMed
 
26. Rannels SR, Yarnell JA, Fisher CS, Fabisiak JP, Rannels DE. Role of laminin in maintenance of type II pneumocyte morphology and function. Am J Physiol. 1987 Dec; 253(6 Pt 1):C835-45.
  View in: PubMed
 
 
Keyword
Last Name
Institution
    
 
 
 
Keywords   
Lung
Pneumonectomy
Carbon-Carbon Ligases
Extracellular Matrix Proteins
Calcium-Binding Proteins
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Co-Authors  
Lanoue, Kathryn
Lynch, Christopher
Pegg, Anthony
See all (3) people
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